Expression of mesenchyme-specific Mch3 antigen in macho-1mRNA-injected partial embryos derived from isolated blastomeres
| . | Partial embryos with Mch3 expression/embryos examined (%) . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Control (mutant) 100 pg . | . | macho-1 . | . | . | . | |||||
| Isolated blastomeres . | . | . | 50 pg . | . | 100 pg . | . | |||||
| BSA | FGF | BSA | FGF | BSA | FGF | ||||||
| A4.1 | 0/19 (0%) | 0/11 (0%) | 17/22 (77%) | 16/19 (84%) | 5/17 (29%) | 10/16 (63%) | |||||
| B4.1 | 11/11 (100%) | 16/16 (100%) | 8/10 (80%) | 7/9 (78%) | 4/13 (31%) | 8/13 (62%) | |||||
| a4.2 | 0/10 (0%) | 0/10 (0%) | 0/10 (0%) | 7/10 (70%) | 0/12 (0%) | 0/10 (0%) | |||||
| b4.2 | 0/10 (0%) | 0/10 (0%) | 0/10 (0%) | 6/10 (60%) | 0/10 (0%) | 2/12 (17%) | |||||
| . | Partial embryos with Mch3 expression/embryos examined (%) . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Control (mutant) 100 pg . | . | macho-1 . | . | . | . | |||||
| Isolated blastomeres . | . | . | 50 pg . | . | 100 pg . | . | |||||
| BSA | FGF | BSA | FGF | BSA | FGF | ||||||
| A4.1 | 0/19 (0%) | 0/11 (0%) | 17/22 (77%) | 16/19 (84%) | 5/17 (29%) | 10/16 (63%) | |||||
| B4.1 | 11/11 (100%) | 16/16 (100%) | 8/10 (80%) | 7/9 (78%) | 4/13 (31%) | 8/13 (62%) | |||||
| a4.2 | 0/10 (0%) | 0/10 (0%) | 0/10 (0%) | 7/10 (70%) | 0/12 (0%) | 0/10 (0%) | |||||
| b4.2 | 0/10 (0%) | 0/10 (0%) | 0/10 (0%) | 6/10 (60%) | 0/10 (0%) | 2/12 (17%) | |||||
Synthetic mRNAs were injected into eggs, then each blastomere was isolated at the eight-cell stage. Isolated blastomeres were treated only with BSA-seawater or with FGF in BSA-seawater. Control mRNA encodes mutant Macho-1 that lacks the zinc-finger domain (Nishida and Sawada, 2001).
Proportions over 50% are indicated in bold.