Parameter . | NaB (10 mM) . | Tubacin (10 μM) . |
---|---|---|
Growth rate (μm/minute) | 5.85±1.53 | 3.96±1.03* |
Shortening rate (μm/minute) | 9.8±4.9 | 6.13±2.34** |
Catastrophe frequency (per second) | 0.07±0.04 | 0.08±0.03 |
Rescue frequency (per second) | 0.12±0.05 | 0.12±0.04 |
% Time in growing | 38±11 | 34±9 |
% Time in pausing | 42±13 | 45±9 |
% Time in shortening | 20±11 | 21±6 |
Parameter . | NaB (10 mM) . | Tubacin (10 μM) . |
---|---|---|
Growth rate (μm/minute) | 5.85±1.53 | 3.96±1.03* |
Shortening rate (μm/minute) | 9.8±4.9 | 6.13±2.34** |
Catastrophe frequency (per second) | 0.07±0.04 | 0.08±0.03 |
Rescue frequency (per second) | 0.12±0.05 | 0.12±0.04 |
% Time in growing | 38±11 | 34±9 |
% Time in pausing | 42±13 | 45±9 |
% Time in shortening | 20±11 | 21±6 |
Parameters of microtubule dynamic instability were measured at the cell periphery of living cells. Values are mean ± s.d. of 21 microtubules. The results of one of the three analogous experiments are presented. *P=0.0001, **P=0.0034, according to the Student's t-test