Analysis of the dve-mutant clones.
Area . | Number of orphan wild-type clones/total number of clones . | Average number of cells in mutant clones . | Average number of cells in the wild-type twin clones . | % cell number (mutant versus wild-type clones) . |
---|---|---|---|---|
A1 | 16/34 (47%) | 6.7 | 13.8 | 49% |
A2 | 0/13 | 10.9 | 16.6 | 66% |
A3 | 6/14 (43%) | 7.6 | 14.8 | 51% |
Area . | Number of orphan wild-type clones/total number of clones . | Average number of cells in mutant clones . | Average number of cells in the wild-type twin clones . | % cell number (mutant versus wild-type clones) . |
---|---|---|---|---|
A1 | 16/34 (47%) | 6.7 | 13.8 | 49% |
A2 | 0/13 | 10.9 | 16.6 | 66% |
A3 | 6/14 (43%) | 7.6 | 14.8 | 51% |
Clones were induced with help of the Flp/FRT system and analysed 48 hours after clone induction by hsFlp. Areas A1-3 are described in Fig. 3E. A1 is the area anterior to the primordium of wing vein 3, A2 extends from vein 3 to vein 4,and A3 is the area posterior to vein 4. The table reveals that the size of the mutant clone in comparison to its wild-type twin is always smaller but varies depending on the area. In A1 and A3 the size of the clone is only around 50%of its wild-type twin, whereas in A2 it is 66%. This suggests that after 48 hours, the mutant cells have gone through one less cell cycle than their wild-type counterpart. Furthermore, although we found that 47% and 43% of the wild-type clones in A1 and A3, respectively, do not have a mutant counterpart,in A2 no orphan wild-type clone was found. These data indicate that Dve is required for the proliferation of all cells in the wing pouch, but the degree of its requirement is dependent on the region.