Quantification of rescue experiments
| . | % of hemisegments (actual number) . | . | |
|---|---|---|---|
| Genotype . | Ddc (EW1 + EW2) . | Corazonin (EW3) . | |
| numb1/numb1 | 3.4 (294) | 0 (54) | |
| numb1/numb1 : spdoG104/+ | 23 (244) | 7 (244) | |
| numb1/numb1 : UASp35/eg-gal4 | 28 (64) | 34 (64) | |
| UAS-NACT/+ : eg-gal4/+ | 0.7 (996) | 0.3 (624) | |
| UAS-NACT/+ : eg-gal4/UASp35 | 97 (64) | 98 (54) | |
| . | % of hemisegments (actual number) . | . | |
|---|---|---|---|
| Genotype . | Ddc (EW1 + EW2) . | Corazonin (EW3) . | |
| numb1/numb1 | 3.4 (294) | 0 (54) | |
| numb1/numb1 : spdoG104/+ | 23 (244) | 7 (244) | |
| numb1/numb1 : UASp35/eg-gal4 | 28 (64) | 34 (64) | |
| UAS-NACT/+ : eg-gal4/+ | 0.7 (996) | 0.3 (624) | |
| UAS-NACT/+ : eg-gal4/UASp35 | 97 (64) | 98 (54) | |
For each genotype, abdominal hemisegments were counted for the number of NB7-3 cells that had at least two Ddc-expressing cells and at least one corazonin cell. The results are presented as a percentage of total number of hemisegments counted. Stage 17 embryos were analyzed in the rescue of the numb1 phenotype. Third instar larvae were analyzed in the rescue of the UAS-NotchACT phenotype. The UAS-NotchACT/+:eg-gal4/UASp35 genotype produces two distinct CNS phenotypes, those that show rescue and those that look like UAS-NotchACT phenotype. Only the fraction of CNS that showed evidence of rescue were counted.