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Table 2.

Statistical analysis of clonality at E8.5 and E10.5

Number of E8.5 hearts*
Number of E10.5 hearts
NObserved with N β-gal-positive clustersExpected with N independent recombination eventsObserved with N β-gal-positive clustersExpected with N independent recombination events
1376  211  
59 108 215 234 
15 120 130 
0.1 60 48 
2×10−3 22 13 
3×10−5 
4×10−7 
  0.1 
⫽     
12   2×10−6 
Number of E8.5 hearts*
Number of E10.5 hearts
NObserved with N β-gal-positive clustersExpected with N independent recombination eventsObserved with N β-gal-positive clustersExpected with N independent recombination events
1376  211  
59 108 215 234 
15 120 130 
0.1 60 48 
2×10−3 22 13 
3×10−5 
4×10−7 
  0.1 
⫽     
12   2×10−6 

We have tested whether multiple clusters within a heart resulted from independent recombination events. The observed distribution of the number of clusters per heart is compared with the expected random distribution, based on the fluctuation test of Luria and Delbrück (see Materials and Methods).

*

Eight positive hearts at E8.5, in which clusters were not clearly distinguishable, were excluded from the observed distribution.

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