sog interacts genetically with genes coding ECM molecules
| . | Interaction with . | . | . | . | |
|---|---|---|---|---|---|
| Gene/allele . | sog (EP7) . | sog (EP11) . | Molecule encoded . | Wing expression pattern . | |
| LamA(25) | EE | EE | Laminin | All wing, veins after apposition | |
| LamA(6-36) | EEE | EEE | |||
| LamA(9-32) | EEE | EEE | |||
| LamA(216) | EE | EE | |||
| DCgl(234) | S | S | Collagen IV a1 | All wing, veins after apposition | |
| Cg25c(GDB) | S | S | |||
| vkg(BLK) | 0 | 0 | Collagen IV a2 | ND | |
| vkg(SAC) | 0 | 0 | |||
| vkg(ICO) | 0 | 0 | |||
| vkg(228) | 0 | 0 | |||
| vkg(177) | 0 | 0 | |||
| mys(1) | EE | EE | βPS integrin | Both wing surfaces; interveins | |
| mys(XB87) | EE | EE | |||
| mys(nj42) | E | E | |||
| mys(nj42)/mys(nj42) | EE | EE | |||
| mys(XR04) | 0 | O | |||
| hs-βPS* | S | S | βPS under control of a hs promoter | NA | |
| mew(498) | S | S | αPS1 integrin | Dorsal wing surface; interveins | |
| mew(M6) | S | S | |||
| if(k27e) | 0 | 0 | αPS2 integrin | Ventral wing surface; interveins | |
| if(B2) | 0 | 0 | |||
| if(3) | 0 | 0 | |||
| scb(1)† | EE | EE | αPS3 integrin | Pupal wing (this report) | |
| scb(2) | EE | EE | |||
| vol(1) | E | E | |||
| vol(2) | E | E | |||
| ds(33k) | 0 | 0 | Cadherin | ND | |
| ds(1) | 0 | 0 | |||
| dally(Gem) | 0 | 0 | Proteoglycan | Imaginal wing disc | |
| dally(P2) | 0 | 0 | |||
| dally(ΔP527) | 0 | 0 | |||
| sas(15) | 0 | 0 | Fibronectin type III repeats | Imaginal discs | |
| fw(1) | E | E | Selectin | ND | |
| tig(X) | 0 | 0 | Ligand for if | ND | |
| tig(A1) | 0 | 0 | |||
| tld(68-62) | 0 | 0 | Metalloprotease | Imaginal wing disc | |
| Df3R slo(3)‡ | EE | EE | |||
| tok(Δ41) | E | E | Metalloprotease | Imaginal wing disc | |
| tkv(1) | EE | EE | Bmp receptor | All wing, stronger at vein border | |
| tkv(8) | S | S | |||
| sax(8) | 0 | 0 | Bmp receptor | Imaginal wing disc | |
| dpp(shv) | EE | EE | Bmp family member | Pupal wing veins | |
| gbb(1) | E | 0 | Bmp family member | Imaginal wing disc | |
| gbb(4) | E | 0 | |||
| rho (ve) | EE | EE | Membrane protein, Egfr signalling | Pupal wing veins | |
| . | Interaction with . | . | . | . | |
|---|---|---|---|---|---|
| Gene/allele . | sog (EP7) . | sog (EP11) . | Molecule encoded . | Wing expression pattern . | |
| LamA(25) | EE | EE | Laminin | All wing, veins after apposition | |
| LamA(6-36) | EEE | EEE | |||
| LamA(9-32) | EEE | EEE | |||
| LamA(216) | EE | EE | |||
| DCgl(234) | S | S | Collagen IV a1 | All wing, veins after apposition | |
| Cg25c(GDB) | S | S | |||
| vkg(BLK) | 0 | 0 | Collagen IV a2 | ND | |
| vkg(SAC) | 0 | 0 | |||
| vkg(ICO) | 0 | 0 | |||
| vkg(228) | 0 | 0 | |||
| vkg(177) | 0 | 0 | |||
| mys(1) | EE | EE | βPS integrin | Both wing surfaces; interveins | |
| mys(XB87) | EE | EE | |||
| mys(nj42) | E | E | |||
| mys(nj42)/mys(nj42) | EE | EE | |||
| mys(XR04) | 0 | O | |||
| hs-βPS* | S | S | βPS under control of a hs promoter | NA | |
| mew(498) | S | S | αPS1 integrin | Dorsal wing surface; interveins | |
| mew(M6) | S | S | |||
| if(k27e) | 0 | 0 | αPS2 integrin | Ventral wing surface; interveins | |
| if(B2) | 0 | 0 | |||
| if(3) | 0 | 0 | |||
| scb(1)† | EE | EE | αPS3 integrin | Pupal wing (this report) | |
| scb(2) | EE | EE | |||
| vol(1) | E | E | |||
| vol(2) | E | E | |||
| ds(33k) | 0 | 0 | Cadherin | ND | |
| ds(1) | 0 | 0 | |||
| dally(Gem) | 0 | 0 | Proteoglycan | Imaginal wing disc | |
| dally(P2) | 0 | 0 | |||
| dally(ΔP527) | 0 | 0 | |||
| sas(15) | 0 | 0 | Fibronectin type III repeats | Imaginal discs | |
| fw(1) | E | E | Selectin | ND | |
| tig(X) | 0 | 0 | Ligand for if | ND | |
| tig(A1) | 0 | 0 | |||
| tld(68-62) | 0 | 0 | Metalloprotease | Imaginal wing disc | |
| Df3R slo(3)‡ | EE | EE | |||
| tok(Δ41) | E | E | Metalloprotease | Imaginal wing disc | |
| tkv(1) | EE | EE | Bmp receptor | All wing, stronger at vein border | |
| tkv(8) | S | S | |||
| sax(8) | 0 | 0 | Bmp receptor | Imaginal wing disc | |
| dpp(shv) | EE | EE | Bmp family member | Pupal wing veins | |
| gbb(1) | E | 0 | Bmp family member | Imaginal wing disc | |
| gbb(4) | E | 0 | |||
| rho (ve) | EE | EE | Membrane protein, Egfr signalling | Pupal wing veins | |
Alleles of genes coding for ECM molecules or their receptors were tested for genetic interactions with sog misexpression lines. Among the Drosophila genes encoding ECM proteins, we tested those for which a wing phenotype and/or expression in the wing had been previously reported. Interactions were scored against the enhancer piracy sog lines EP7 and EP11 (Yu et al., 1996),and classified as no interaction (0), enhancement (E) or suppression (S) of the sogEP vein truncation phenotype. In the case of enhancer mutations, the number of Es indicates the strength of the interaction. sogEP7 induces ectopic expression of sog in vein domains during pupal development. Interacting alleles were also crossed to enhancer piracy lines EP9, EP8, EP2 and EP3. In all such cases, we observed consistent types of interactions. Unless cited,modifications of the sogEP phenotype were similar for both the L4/L5 truncation and L2 wandering phenotypes.
The strongest effects were observed when pupae were heat shocked at 4-8 hours after fertilization and 20-28 hours after fertilization, which coincide with periods of apposition between wing surfaces(Fristrom et al., 1993). Phenotypes were compared with those observed in sogEP7/+flies heat shocked under the same conditions.
scb only enhances the vein truncation phenotype.
The deficiency Df 3R slo3 uncovers tld and tok.
NA, not applicable; ND, not determined or not analyzed through all stages.