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Table 1.

Autonomous production of definitive myeloid cells in the VDA

Genetic backgroundMarkerUncaging positionStage examinedCo-stained cells in the uncaged regionCo-stained cells in the PBI
Wild type L-plastin Anterior ICM 2 dpf 7/24* 4/24* 
   3 dpf 9/25 6/25 
 lyc Anterior ICM 2 dpf 7/22 3/22 
Genetic backgroundMarkerUncaging positionStage examinedCo-stained cells in the uncaged regionCo-stained cells in the PBI
Wild type L-plastin Anterior ICM 2 dpf 7/24* 4/24* 
   3 dpf 9/25 6/25 
 lyc Anterior ICM 2 dpf 7/22 3/22 

The ratio indicates the number of embryos containing flu/myeloid marker co-stained cells in the corresponding region versus total number of uncaged embryos.

*

Three embryos contain co-stained cells in both uncaged region and PBI.

Four embryos contain co-stained cells in both uncaged region and PBI.

Two embryos contain co-stained cells in both uncaged region and PBI.

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