Table 1.

GSLs from the germ line are necessary for EGFR signaling

A
 

 

 

 
 Phenotype (%)
 
  
Genotype
 
Complete fusion
 
Partial fusion
 
Wild type
 
brn1.6P6FRT101;e22cGAL4FLP (n=500) 0.2±0.4 0.6±0.9 99.2±0.8 
egh62d18FRT101;e22cGAL4FLP (n=500)
 
0±0
 
1.6±1.1
 
98.4±1.1
 
B
 

 

 

 
 Phenotype (%)
 
  
Genotype
 
Complete or partial fusion
 

 
Wild type
 
brn1.6P6FRT101/OvoDFRT101;hsFLP(n=213) 34.3  65.7 
egh62d18FRT101/OvoDFRT101;hsFLP (n=87)
 
42.5
 

 
57.5
 
C
 

 

 

 
 Phenotype (%)
 
  
Genotype
 
Complete fusion
 
Partial fusion
 
Wild type
 
brnfs107 (n=300) 83.9±9.2 14.9±8.1 1.2±1.4 
brnfs107;;nosGAL4/UASPbrn (n=300) 0±0 0.7±0.6 99.3±0.6 
A
 

 

 

 
 Phenotype (%)
 
  
Genotype
 
Complete fusion
 
Partial fusion
 
Wild type
 
brn1.6P6FRT101;e22cGAL4FLP (n=500) 0.2±0.4 0.6±0.9 99.2±0.8 
egh62d18FRT101;e22cGAL4FLP (n=500)
 
0±0
 
1.6±1.1
 
98.4±1.1
 
B
 

 

 

 
 Phenotype (%)
 
  
Genotype
 
Complete or partial fusion
 

 
Wild type
 
brn1.6P6FRT101/OvoDFRT101;hsFLP(n=213) 34.3  65.7 
egh62d18FRT101/OvoDFRT101;hsFLP (n=87)
 
42.5
 

 
57.5
 
C
 

 

 

 
 Phenotype (%)
 
  
Genotype
 
Complete fusion
 
Partial fusion
 
Wild type
 
brnfs107 (n=300) 83.9±9.2 14.9±8.1 1.2±1.4 
brnfs107;;nosGAL4/UASPbrn (n=300) 0±0 0.7±0.6 99.3±0.6 

Follicle cell (A) and germ line (B) clones were generated for a lethal allele of egh or brn. Only eggs derived from germ line clones show a fusion of their RAs. Note that in B, the s.d. is not included because females from these genotypes laid very few eggs, precluding statistical analysis. (C) The appendage fusion phenotype of the brnviable allele is rescued by brn expression in the germ line.

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