Meiotic defects in CTCF-depleted oocytes
| . | . | . | . | . | 16 hours* . | . | |
|---|---|---|---|---|---|---|---|
| Line . | Genotype . | 0 hours* %SN-type (n) . | 1 hour* % GVBD(n) . | 2 hours* % GVBD(n) . | % Ana/telo 1 (n) . | % PBE (n) . | |
| 1 | Ntg | 68.7 (131) | 26.0 (50) | 76.0 (50) | 9.5 (63) | 55.6 (63) | |
| Tg | 48.0 (98) | 2.0 (50) | 74.0 (50) | 16.1 (62) | 32.3 (62) | ||
| P<0.01 | P<0.001 | P=1 | P=0.3 | P<0.05 | |||
| 12 | Ntg | 64.5 (110) | ND | ND | 3.2 (62) | 38.7 (62) | |
| Tg | 57.6 (92) | ND | ND | 6.2 (81) | 24.7 (81) | ||
| P=0.384 | P=0.7 | P=0.099 | |||||
| 21 | Ntg | 81.5 (119) | ND | ND | 4.8 (62) | 58.1 (62) | |
| Tg | 64.9 (111) | ND | ND | 12.1 (66) | 28.8 (66) | ||
| P<0.01 | P=0.21 | P<0.01 | |||||
| . | . | . | . | . | 16 hours* . | . | |
|---|---|---|---|---|---|---|---|
| Line . | Genotype . | 0 hours* %SN-type (n) . | 1 hour* % GVBD(n) . | 2 hours* % GVBD(n) . | % Ana/telo 1 (n) . | % PBE (n) . | |
| 1 | Ntg | 68.7 (131) | 26.0 (50) | 76.0 (50) | 9.5 (63) | 55.6 (63) | |
| Tg | 48.0 (98) | 2.0 (50) | 74.0 (50) | 16.1 (62) | 32.3 (62) | ||
| P<0.01 | P<0.001 | P=1 | P=0.3 | P<0.05 | |||
| 12 | Ntg | 64.5 (110) | ND | ND | 3.2 (62) | 38.7 (62) | |
| Tg | 57.6 (92) | ND | ND | 6.2 (81) | 24.7 (81) | ||
| P=0.384 | P=0.7 | P=0.099 | |||||
| 21 | Ntg | 81.5 (119) | ND | ND | 4.8 (62) | 58.1 (62) | |
| Tg | 64.9 (111) | ND | ND | 12.1 (66) | 28.8 (66) | ||
| P<0.01 | P=0.21 | P<0.01 | |||||
SN-type, surrounded nucleolus-type GV oocytes.
GVBD, germinal vesicle breakdown.
Ana/telo 1, anaphase or telophase of meiosis 1.
PBE, polar body extrusion.
Ntg, non-transgenic; Tg, transgenic.
n, number of oocytes.
ND, not determined.
Germinal vesicle (GV) oocytes were cultured for the indicated number of hours.