Validation of gene misregulation by real-time PCR
| . | Microarray Line 1 . | Real-time PCR . | . | . | ||
|---|---|---|---|---|---|---|
| Gene . | . | Line 1 . | Line 12 . | Line 21 . | ||
| Ctcf | 0.03 | 0.0094±0.0004 | 0.0094±0.003 | 0.0092±0.002 | ||
| Pim1 | 0.48 | 0.62±0.06 | 0.54±0.26 | 0.38±0.06 | ||
| Cbfa2t1h | 0.55 | 0.61±0.06 | 0.62±0.15 | 0.69±0.08 | ||
| Gtl2 | 0.55 | 0.53±0.08 | 0.51±0.02 | 0.55±0.05 | ||
| Grb10 | 0.84 | 0.78±0.04 | 0.61±0.15 | 0.68±0.24 | ||
| Myc | ND | 1.05±0.12 | 0.91±0.35 | 0.86±0.18 | ||
| Boris | ND | A:A | A:A | A:A | ||
| Slc22a18 | 1.77 | 2.80±0.18 | 1.88±0.50 | 1.94±0.21 | ||
| Phlda2 | 1.86 | 2.18±0.23 | 1.55±0.15 | 2.16±1.15 | ||
| Fcgr1 | 35.9 | 75.0±6.1 | 76.4±23.9 | 73.0±10.8 | ||
| Tlr1 | 58.0 | 1:A | 1:A | 1:A | ||
| . | Microarray Line 1 . | Real-time PCR . | . | . | ||
|---|---|---|---|---|---|---|
| Gene . | . | Line 1 . | Line 12 . | Line 21 . | ||
| Ctcf | 0.03 | 0.0094±0.0004 | 0.0094±0.003 | 0.0092±0.002 | ||
| Pim1 | 0.48 | 0.62±0.06 | 0.54±0.26 | 0.38±0.06 | ||
| Cbfa2t1h | 0.55 | 0.61±0.06 | 0.62±0.15 | 0.69±0.08 | ||
| Gtl2 | 0.55 | 0.53±0.08 | 0.51±0.02 | 0.55±0.05 | ||
| Grb10 | 0.84 | 0.78±0.04 | 0.61±0.15 | 0.68±0.24 | ||
| Myc | ND | 1.05±0.12 | 0.91±0.35 | 0.86±0.18 | ||
| Boris | ND | A:A | A:A | A:A | ||
| Slc22a18 | 1.77 | 2.80±0.18 | 1.88±0.50 | 1.94±0.21 | ||
| Phlda2 | 1.86 | 2.18±0.23 | 1.55±0.15 | 2.16±1.15 | ||
| Fcgr1 | 35.9 | 75.0±6.1 | 76.4±23.9 | 73.0±10.8 | ||
| Tlr1 | 58.0 | 1:A | 1:A | 1:A | ||
Values are transgenic expression relative to non-transgenic expression±s.e.m.
1:A, detected in transgenic oocytes but absent from non-transgenic oocytes.
A:A, absent from both transgenic and non-transgenic oocytes.
ND, not determined