Skip to Main Content
Table 1.

Validation of gene misregulation by real-time PCR

Microarray Line 1Real-time PCR
GeneLine 1Line 12Line 21
Ctcf 0.03 0.0094±0.0004 0.0094±0.003 0.0092±0.002 
Pim1 0.48 0.62±0.06 0.54±0.26 0.38±0.06 
Cbfa2t1h 0.55 0.61±0.06 0.62±0.15 0.69±0.08 
Gtl2 0.55 0.53±0.08 0.51±0.02 0.55±0.05 
Grb10 0.84 0.78±0.04 0.61±0.15 0.68±0.24 
Myc ND 1.05±0.12 0.91±0.35 0.86±0.18 
Boris ND A:A A:A A:A 
Slc22a18 1.77 2.80±0.18 1.88±0.50 1.94±0.21 
Phlda2 1.86 2.18±0.23 1.55±0.15 2.16±1.15 
Fcgr1 35.9 75.0±6.1 76.4±23.9 73.0±10.8 
Tlr1 58.0 1:A 1:A 1:A 
Microarray Line 1Real-time PCR
GeneLine 1Line 12Line 21
Ctcf 0.03 0.0094±0.0004 0.0094±0.003 0.0092±0.002 
Pim1 0.48 0.62±0.06 0.54±0.26 0.38±0.06 
Cbfa2t1h 0.55 0.61±0.06 0.62±0.15 0.69±0.08 
Gtl2 0.55 0.53±0.08 0.51±0.02 0.55±0.05 
Grb10 0.84 0.78±0.04 0.61±0.15 0.68±0.24 
Myc ND 1.05±0.12 0.91±0.35 0.86±0.18 
Boris ND A:A A:A A:A 
Slc22a18 1.77 2.80±0.18 1.88±0.50 1.94±0.21 
Phlda2 1.86 2.18±0.23 1.55±0.15 2.16±1.15 
Fcgr1 35.9 75.0±6.1 76.4±23.9 73.0±10.8 
Tlr1 58.0 1:A 1:A 1:A 

Values are transgenic expression relative to non-transgenic expression±s.e.m.

1:A, detected in transgenic oocytes but absent from non-transgenic oocytes.

A:A, absent from both transgenic and non-transgenic oocytes.

ND, not determined

Close Modal

or Create an Account

Close Modal
Close Modal