. | . | . | trxG alleles . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TRE/PREs* . | Δ region C module . | Transgenic line . | trxB11 . | Asx3 . | ash122 . | ash112 . | brm2 . | brm5 . | |||||
C1 TRE: trx | ΔC1 | 1-11-42 | − | − | + | + | + | + | |||||
ΔC1 | 1-11-51 | − | − | + | + | + | + | ||||||
ΔC1 | 1-11-17 | − | − | + | + | + | + | ||||||
ΔC1 | 1-11-61 | − | − | + | + | + | + | ||||||
ΔC1 | 1-11-11 | − | NT | + | + | + | + | ||||||
ΔC1 | 1-11-10 | − | NT | + | + | + | + | ||||||
C2 PRE: Scm | ΔC2 | 14-7-42 | + | + | − | − | − | − | |||||
ΔC2 | 14-7-8 | + | NT | − | − | − | − | ||||||
ΔC2 | 14-7-3 | + | NT | − | − | − | − | ||||||
ΔC2 | 14-7-6 | + | NT | − | − | − | − | ||||||
ΔC2 | 14-7-7 | + | NT | + | − | − | − | ||||||
C3 PRE: Psc, Pcl, Scm, pho | ΔC3 | 2-3-18 | + | + | + | + | + | + | |||||
ΔC3 | 2-3-13 | + | + | + | − | + | + | ||||||
ΔC3 | 2-22-02 | + | + | + | + | + | + | ||||||
ΔC3 | 2-3-84 | + | NT | − | − | + | + | ||||||
ΔC3 | 2-22-85 | + | NT | − | + | + | + |
. | . | . | trxG alleles . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TRE/PREs* . | Δ region C module . | Transgenic line . | trxB11 . | Asx3 . | ash122 . | ash112 . | brm2 . | brm5 . | |||||
C1 TRE: trx | ΔC1 | 1-11-42 | − | − | + | + | + | + | |||||
ΔC1 | 1-11-51 | − | − | + | + | + | + | ||||||
ΔC1 | 1-11-17 | − | − | + | + | + | + | ||||||
ΔC1 | 1-11-61 | − | − | + | + | + | + | ||||||
ΔC1 | 1-11-11 | − | NT | + | + | + | + | ||||||
ΔC1 | 1-11-10 | − | NT | + | + | + | + | ||||||
C2 PRE: Scm | ΔC2 | 14-7-42 | + | + | − | − | − | − | |||||
ΔC2 | 14-7-8 | + | NT | − | − | − | − | ||||||
ΔC2 | 14-7-3 | + | NT | − | − | − | − | ||||||
ΔC2 | 14-7-6 | + | NT | − | − | − | − | ||||||
ΔC2 | 14-7-7 | + | NT | + | − | − | − | ||||||
C3 PRE: Psc, Pcl, Scm, pho | ΔC3 | 2-3-18 | + | + | + | + | + | + | |||||
ΔC3 | 2-3-13 | + | + | + | − | + | + | ||||||
ΔC3 | 2-22-02 | + | + | + | + | + | + | ||||||
ΔC3 | 2-3-84 | + | NT | − | − | + | + | ||||||
ΔC3 | 2-22-85 | + | NT | − | + | + | + |
The effects of trxG alleles trxB11, ash112,ash122, brm2, brm5 and the ETP allele Asx3 on expression of N and ΔC transgenes containing deletions of the TRE or PRE (see Fig. 1). Adult flies from each line were crossed to each of the tested alleles and the eye color of the progeny was compared to the parent line. A decrease in eye color expression in the progeny was designated (+), and no change in eye color expression designated (−) as suggestive of a deleted response element for a given trxG or ETP protein. The transgenes containing the deleted C1 and C2 elements showed no further change in expression in the trx and Asx or ash1 and brm mutants,respectively, suggesting that response elements for Trx and Asx reside in the C1 element, and that response elements for Ash1 and Brm reside in the C2 element. The response element for Ash1 may extend into the C3 element.
Nt, not tested.
As previously identified (Tillib et al., 1999).