. | 2-deoxy-D-glucose uptake (% of basal in absence of inhibitor) . | . | 3-O-methylglucose uptake (% of basal in absence of inhibitor) . | . | ||
---|---|---|---|---|---|---|
Treatment . | Basal . | 5 mM azide . | Basal . | 500 μM AICAR . | ||
No inhibitora | 100.0±6.5 | 470.7±24.0 | 100.0±41.9 | 285.5±41.5 | ||
400 μM L-NMMAa | 68.3±3.6 | 324.6±5.6* | 41.1±11.6 | 209.3±23.7 | ||
No inhibitorb | 100.0±26.2 | 761.4±13.8 | 100.0±51.1 | 438.9±133.8 | ||
2 mM L-NAMEb | 77.4±6.1 | 554.7±7.1* | 138.9±55.7 | 379.5±23.0 |
. | 2-deoxy-D-glucose uptake (% of basal in absence of inhibitor) . | . | 3-O-methylglucose uptake (% of basal in absence of inhibitor) . | . | ||
---|---|---|---|---|---|---|
Treatment . | Basal . | 5 mM azide . | Basal . | 500 μM AICAR . | ||
No inhibitora | 100.0±6.5 | 470.7±24.0 | 100.0±41.9 | 285.5±41.5 | ||
400 μM L-NMMAa | 68.3±3.6 | 324.6±5.6* | 41.1±11.6 | 209.3±23.7 | ||
No inhibitorb | 100.0±26.2 | 761.4±13.8 | 100.0±51.1 | 438.9±133.8 | ||
2 mM L-NAMEb | 77.4±6.1 | 554.7±7.1* | 138.9±55.7 | 379.5±23.0 |
Clone 9 cells were treated with or without 400 μM L-NMMA or 2 mM L-NAME for 60 minutes. During the last 30 minutes of this period they were exposed either to 5 mM sodium azide or 500 μM AICAR or exposed to neither agent(basal). The uptake of [3H]3-O-methyl-D-glucose or[3H]2-deoxy-D-glucose was then measured as described in the Materials and Methods. a,bSeparate batches of cells, which exhibited slightly differing fold-stimulations of transport by azide and AICAR, were used for experiments with L-NMMA and L-NAME. The results shown are the means±s.e.m for three experiments; *this value is significantly different (P<0.05) from azide-stimulated uptake rate in the absence of the inhibitor.