The number of E7.0-E10.5 embryos of different genotypes derived from Dkk1+/−;Wnt3+/−intercross
| . | Number of embryos of each genotype*(number displaying abnormalities) . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Dkk1+/+ . | Dkk1+/+ . | Dkk1+/− . | Dkk1+/− . | Dkk1+/+ . | Dkk1+/− . | Dkk1−/− . | Dkk1−/− . | Dkk1−/− . | . | |||||||||
| Age (E) . | Wnt3+/+ . | Wnt3+/− . | Wnt3+/+ . | Wnt3+/− . | Wnt3−/− . | Wnt3−/− . | Wnt3+/+ . | Wnt3+/− . | Wnt3−/− . | Total . | |||||||||
| 7.0-8.0 | 1 (0) | 3 (0) | 1 (1) | 8 (8) | 5 (5) | 4 (4) | 1 (1) | 4 (4) | 3 (3) | 30 | |||||||||
| 8.0-9.0 | 4 (0) | 12 (1) | 4 (1) | 8 (6) | 7 (7) | 6 (6) | 2 (2) | 3 (3) | 2 (2) | 48 | |||||||||
| 9.5 | 12 (0) | 24 (1) | 17 (1) | 45 (31) | 12 (12) | 12 (11) | 4 (4) | 10 (10) | 3 (3) | 139 | |||||||||
| Total number | 17 (0) | 39 (2) | 22 (3) | 61 (45) | 24 (24) | 22 (21) | 7 (7) | 17 (17) | 8 (8) | 217 | |||||||||
| % Abnormal | 0 | 5 | 14 | 74 | 100 | 95 | 100 | 100 | 100 | ||||||||||
| 10.5 | 11 (0) | 19 (1) | 21 (2) | 26 (3) | 0 | 0 | 7 (7) | 22 (16) | 0 | 106 | |||||||||
| % Abnormal | 0 | 5 | 10 | 12 | N/A | N/A | 100 | 73 | N/A | ||||||||||
| . | Number of embryos of each genotype*(number displaying abnormalities) . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Dkk1+/+ . | Dkk1+/+ . | Dkk1+/− . | Dkk1+/− . | Dkk1+/+ . | Dkk1+/− . | Dkk1−/− . | Dkk1−/− . | Dkk1−/− . | . | |||||||||
| Age (E) . | Wnt3+/+ . | Wnt3+/− . | Wnt3+/+ . | Wnt3+/− . | Wnt3−/− . | Wnt3−/− . | Wnt3+/+ . | Wnt3+/− . | Wnt3−/− . | Total . | |||||||||
| 7.0-8.0 | 1 (0) | 3 (0) | 1 (1) | 8 (8) | 5 (5) | 4 (4) | 1 (1) | 4 (4) | 3 (3) | 30 | |||||||||
| 8.0-9.0 | 4 (0) | 12 (1) | 4 (1) | 8 (6) | 7 (7) | 6 (6) | 2 (2) | 3 (3) | 2 (2) | 48 | |||||||||
| 9.5 | 12 (0) | 24 (1) | 17 (1) | 45 (31) | 12 (12) | 12 (11) | 4 (4) | 10 (10) | 3 (3) | 139 | |||||||||
| Total number | 17 (0) | 39 (2) | 22 (3) | 61 (45) | 24 (24) | 22 (21) | 7 (7) | 17 (17) | 8 (8) | 217 | |||||||||
| % Abnormal | 0 | 5 | 14 | 74 | 100 | 95 | 100 | 100 | 100 | ||||||||||
| 10.5 | 11 (0) | 19 (1) | 21 (2) | 26 (3) | 0 | 0 | 7 (7) | 22 (16) | 0 | 106 | |||||||||
| % Abnormal | 0 | 5 | 10 | 12 | N/A | N/A | 100 | 73 | N/A | ||||||||||
Embryos of three age groups (E7.0-8.0, E8.0-9.0 and E9.0-10.0) were collected. The number of embryos of each genotype is shown, followed by the number (in parentheses) that displayed abnormal phenotype; the percentage of abnormal embryos in each genotype class is shown in the last row of each intercross.
N/A, not applicable as no embryos of this genotype were found.
ϰ2 analysis was performed on the frequency of each genotype across all age groups, but genotypes were not present at the expected Mendelian frequency (ϰ2=30.5, significant difference at P<0.05) due to the loss of Wnt3-null embryos, which were arrested at gastrulation.