Levels of Dkk1 and Wnt3 expression in embryos of (A) Wnt3 and (B) Dkk1;Wnt3 crosses as determined by quantitative RT-PCR
| . | . | Expression level† . | . | |
|---|---|---|---|---|
| Genotype of embryo . | . | Dkk1 (n) . | Wnt3 (n) . | |
| A. Wnt3+/− × Wnt3+/− | ||||
| Dkk1+/+ | Wnt3+/+ | 99032±10365 (7) [100%] | 6039±1294 (7) [100%] | |
| Dkk1+/+ | Wnt3+/− | 101383±12083 (6) [102%] | 5111±1995 (6) [84%] | |
| Dkk1+/+ | Wnt3−/− | 5552±1871** (8) [5.6%] | 118±60** (8) [1.9%] | |
| B. Dkk1+/−;Wnt3+/−×Dkk1+/−;Wnt3+/− | ||||
| Dkk1+/+ | Wnt3+/+ | 2265±275 (8) [100%] | 306±86 (8) [100%] | |
| Dkk1+/+ | Wnt3+/− | 2038±449 (9) [90%] | 175±49 (9) [57%] | |
| Dkk1+/− | Wnt3+/+ | 1617±317 (5) [71%] | 201±110 (5) [66%] | |
| Dkk1+/− | Wnt3+/− | 1027±167* (7) [45%] | 149±34 (7) [49%] | |
| . | . | Expression level† . | . | |
|---|---|---|---|---|
| Genotype of embryo . | . | Dkk1 (n) . | Wnt3 (n) . | |
| A. Wnt3+/− × Wnt3+/− | ||||
| Dkk1+/+ | Wnt3+/+ | 99032±10365 (7) [100%] | 6039±1294 (7) [100%] | |
| Dkk1+/+ | Wnt3+/− | 101383±12083 (6) [102%] | 5111±1995 (6) [84%] | |
| Dkk1+/+ | Wnt3−/− | 5552±1871** (8) [5.6%] | 118±60** (8) [1.9%] | |
| B. Dkk1+/−;Wnt3+/−×Dkk1+/−;Wnt3+/− | ||||
| Dkk1+/+ | Wnt3+/+ | 2265±275 (8) [100%] | 306±86 (8) [100%] | |
| Dkk1+/+ | Wnt3+/− | 2038±449 (9) [90%] | 175±49 (9) [57%] | |
| Dkk1+/− | Wnt3+/+ | 1617±317 (5) [71%] | 201±110 (5) [66%] | |
| Dkk1+/− | Wnt3+/− | 1027±167* (7) [45%] | 149±34 (7) [49%] | |
*P<0.01, **P<0.01 by Student's t-test compared with Dkk1+/+;Wnt3+/+ level. The difference in the absolute values of the expression level between datasets A and B is due to the use of RT-PCR machines with different settings. However, each set is internally consistent as the samples were analyzed under similar conditions using the same instrument.
Expression level: mean±s.e. arbitrary units normalized relative to GAPDH [%].