The expression of XNR1 in the lateral plate mesoderm (LPM)following ectopic expression of wild-type or mutant XCR2
| . | . | XNR1 expression in the LPM . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | Injection side . | No signal . | Left (normal) . | Bilateral . | Right (invert) . | Total . | |||
| pCS-EGFP | Left | 6% | 94% | 0% | 0% | 32 | |||
| pCS-EGFP | Right | 0% | 100% | 0% | 0% | 32 | |||
| pCS-XCR2 | Left | 0% | 92% | 8% | 0% | 26 | |||
| pCS-XCR2 | Right | 5% | 0% | 55% | 40% | 20 | |||
| pCS-XCR2 mEGF | Left | 0% | 96% | 4% | 0% | 25 | |||
| pCS-XCR2 mEGF | Right | 3% | 97% | 0% | 0% | 33 | |||
| pCS-XCR2 mCFC | Left | 0% | 95% | 5% | 0% | 20 | |||
| pCS-XCR2 mCFC | Right | 0 | 96% | 4% | 0 | 26 | |||
| . | . | XNR1 expression in the LPM . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | Injection side . | No signal . | Left (normal) . | Bilateral . | Right (invert) . | Total . | |||
| pCS-EGFP | Left | 6% | 94% | 0% | 0% | 32 | |||
| pCS-EGFP | Right | 0% | 100% | 0% | 0% | 32 | |||
| pCS-XCR2 | Left | 0% | 92% | 8% | 0% | 26 | |||
| pCS-XCR2 | Right | 5% | 0% | 55% | 40% | 20 | |||
| pCS-XCR2 mEGF | Left | 0% | 96% | 4% | 0% | 25 | |||
| pCS-XCR2 mEGF | Right | 3% | 97% | 0% | 0% | 33 | |||
| pCS-XCR2 mCFC | Left | 0% | 95% | 5% | 0% | 20 | |||
| pCS-XCR2 mCFC | Right | 0 | 96% | 4% | 0 | 26 | |||
40 pg of plasmids were injected with 0.5 ng of EGFP RNA into the marginal region of one blastomere at the two-cell stage. Embryos were sorted by EGFP fluorescence at stage 20-21, fixed at stage 24-25 and analyzed for XNR1 expression by in situ hybridization.