The effect of XCR2 depletion on expression of XNR1 in the posterior archenteron roof and in the LPM
| . | . | XNR1 expression region . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Posterior archenteron roof . | LPM . | . | . | . | . | ||||
| . | Stage . | Bilateral . | No signal . | Left (normal) . | Bilateral . | Right (invert) . | Total . | ||||
| SC MO | 15 | 100% | 25 | ||||||||
| XCR2 MO | 15 | 100% | 25 | ||||||||
| SC MO | 20 | 100% | 0% | 92% | 8% | 0% | 25 | ||||
| XCR2 MO | 20 | 100% | 100% | 0% | 0% | 0% | 26 | ||||
| . | . | XNR1 expression region . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Posterior archenteron roof . | LPM . | . | . | . | . | ||||
| . | Stage . | Bilateral . | No signal . | Left (normal) . | Bilateral . | Right (invert) . | Total . | ||||
| SC MO | 15 | 100% | 25 | ||||||||
| XCR2 MO | 15 | 100% | 25 | ||||||||
| SC MO | 20 | 100% | 0% | 92% | 8% | 0% | 25 | ||||
| XCR2 MO | 20 | 100% | 100% | 0% | 0% | 0% | 26 | ||||
20 ng of MOs were injected into the marginal region of both blastomeres at the two-cell stage. Embryos were fixed at stage 15 or 20, as indicated, and prepared for analysis of XNR1 expression by in situ hybridization.
SC MO, standard control MO.