. | Amount of MO (ng) . | Heart . | . | . | Gut . | . | . | . | . | . | . | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | . | Normal . | Un . | Mirror . | Normal (RO/CCW) . | RO/CW . | Un . | LO/CCW . | Mirror (LO/CW) . | Other . | Total . | ||||||
Uninjected | 95% | 2% | 0% | 95% | 1% | 0% | 1% | 0% | 3% | 129 | |||||||
SC MO | 20 | 85% | 4% | 6% | 88% | 3% | 0% | 4% | 0% | 4% | 67 | ||||||
SC MO | 50 | 90% | 1% | 1% | 89% | 3% | 1% | 0% | 0% | 7% | 72 | ||||||
XCR2 MO | 20 | 55% | 4% | 23% | 27% | 14% | 4% | 23% | 14% | 18% | 56 | ||||||
XCR2 MO | 50 | 39% | 12% | 37% | 24% | 22% | 12% | 18% | 12% | 12% | 67 |
. | Amount of MO (ng) . | Heart . | . | . | Gut . | . | . | . | . | . | . | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | . | Normal . | Un . | Mirror . | Normal (RO/CCW) . | RO/CW . | Un . | LO/CCW . | Mirror (LO/CW) . | Other . | Total . | ||||||
Uninjected | 95% | 2% | 0% | 95% | 1% | 0% | 1% | 0% | 3% | 129 | |||||||
SC MO | 20 | 85% | 4% | 6% | 88% | 3% | 0% | 4% | 0% | 4% | 67 | ||||||
SC MO | 50 | 90% | 1% | 1% | 89% | 3% | 1% | 0% | 0% | 7% | 72 | ||||||
XCR2 MO | 20 | 55% | 4% | 23% | 27% | 14% | 4% | 23% | 14% | 18% | 56 | ||||||
XCR2 MO | 50 | 39% | 12% | 37% | 24% | 22% | 12% | 18% | 12% | 12% | 67 |
MOs were injected into the marginal region of both blastomeres at the two-cell stage. Embryos were counted at stage 45-46. Embryos which had other defects besides left-right axis (Other) were not counted in either heart and gut morphologies.
SC MO, standard control MO; Mirror, reversed left-right asymmetry; Un,undistinguishable left-right morphology; RO/CCW, right-origin/counterclockwise gut; RO/CW, right-origin/clockwise gut; LO/CCW, left-origin/counterclockwise gut; LO/CW, left-origin/clockwise gut; Other, other defects besides left-right axis.