Skip to Main Content
Table 1.

Quantitation of the dorsal open phenotype in epistatic analysis

Expected if fully rescued
Observed
Total
Cross% DC% DO% DC% DO% SHn
DdokPG155/DdokPG155(GLC)X+/Y 50 50 52 48 486 
DdokPG155/DdokPG155(GLC)X+/Y;hs-junasp/+; +/+ (+hs) 75 25 50 31 19 100 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; pucE69/+ 75 25 50 29 21 601 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; hs-shark-10/hs-shark-10 (+hs) 100 50 10 40 392 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; hs-shark-K698R/hs-shark-K698R (+hs) 100 53 47 400 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; hs-Src42A22.3/hs-Src42A22.3 (+hs) 100 56 44 184 
Expected if fully rescued
Observed
Total
Cross% DC% DO% DC% DO% SHn
DdokPG155/DdokPG155(GLC)X+/Y 50 50 52 48 486 
DdokPG155/DdokPG155(GLC)X+/Y;hs-junasp/+; +/+ (+hs) 75 25 50 31 19 100 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; pucE69/+ 75 25 50 29 21 601 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; hs-shark-10/hs-shark-10 (+hs) 100 50 10 40 392 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; hs-shark-K698R/hs-shark-K698R (+hs) 100 53 47 400 
DdokPG155/DdokPG155(GLC)X+/Y; +/+; hs-Src42A22.3/hs-Src42A22.3 (+hs) 100 56 44 184 

Embryos were collected and aged for 24 hours after egg lay. In all heat-shock (hs) experiments, 5- to 8-hour staged embryos were heat shocked at 37°C for a period of 30 minutes for the induction of the transgene. The total number of embryos and hatched larvae (n) was determined by counting in Hoyers solution. Cuticles were prepared from unhatched embryos,and the number of embryos with dorsal closed (DC), dorsal open (DO) and small hole (SH) cuticles enumerated. The % DC group includes hatched larvae as well as embryos with DC articles.

Close Modal

or Create an Account

Close Modal
Close Modal