Correlation between somitic Fgf10 expression and mammary development
| . | . | . | Development of mammary placodes . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype . | Fgf10 expression in somitic subdomains . | Histology of mammary line at E11.5 (±45 seconds) . | 1 . | 2 . | 3 . | 4 . | 5 . | ||||
| Wild type | Entire somites 11-18, high hypaxial and epaxial expression | Cylindrical s.g., cylindrical s.i., covered with periderm | + | + | + | + | + | ||||
| Pax3ILZ/ILZ | No hypaxial and epaxial expression, normal central expression in somites 11-18 | Cylindrical s.g., reduced cylindrical s.i., covered with periderm | + | + | Late | + | + | ||||
| Fgf10–/– | None | Cuboidal s.g., no s.i., periderm impaired | – | – | – | + | – | ||||
| Fgfr2b–/– | As wild type (absent receptor for somitic FGFf10) | As Fgf10–/– | – | – | – | + | – | ||||
| Gli3Xt-J/Xt-J | Only hypaxial somites 11-16, low expression levels | Cylindrical s.g., no s.i., covered with periderm | + | + | – | + | – | ||||
| Fgf10hypomorph | Pattern as in wild type, but lower expression levels | As Gil3Xt-J/Xt-J | + | + | – | + | + | ||||
| . | . | . | Development of mammary placodes . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype . | Fgf10 expression in somitic subdomains . | Histology of mammary line at E11.5 (±45 seconds) . | 1 . | 2 . | 3 . | 4 . | 5 . | ||||
| Wild type | Entire somites 11-18, high hypaxial and epaxial expression | Cylindrical s.g., cylindrical s.i., covered with periderm | + | + | + | + | + | ||||
| Pax3ILZ/ILZ | No hypaxial and epaxial expression, normal central expression in somites 11-18 | Cylindrical s.g., reduced cylindrical s.i., covered with periderm | + | + | Late | + | + | ||||
| Fgf10–/– | None | Cuboidal s.g., no s.i., periderm impaired | – | – | – | + | – | ||||
| Fgfr2b–/– | As wild type (absent receptor for somitic FGFf10) | As Fgf10–/– | – | – | – | + | – | ||||
| Gli3Xt-J/Xt-J | Only hypaxial somites 11-16, low expression levels | Cylindrical s.g., no s.i., covered with periderm | + | + | – | + | – | ||||
| Fgf10hypomorph | Pattern as in wild type, but lower expression levels | As Gil3Xt-J/Xt-J | + | + | – | + | + | ||||
For each mutant used in this study (column 1), the Fgf10expression pattern in the somites (column 2) and the histology of the mammary line at around E11.5 (column 3) are described. The formation (+) or the absence (–) of each of the five placode pairs is also indicated (columns 4-8)
Abbreviations: s.g., stratum germinativum; s.i., stratum intermedium