Recombinant FGF10 rescues placode formation in vitro
| . | Genotype and treatment . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of glands . | Control . | Control + BSA . | Control + Fgf10 . | Fgf10–/– + BSA . | Fgf10–/– + Fgf10 . | Gli3Xt-JIXt-J . | Gli3Xt-JIXt-J + BSA . | Gli3Xt-JIXt-J + Fgf10 . | ||||||||
| 0 | 5 | 4 | 27 | 2 | 10 | 1 | 0 | 6 | ||||||||
| 1 | 0 | 6 | 4 | 0 | 6 | 0 | 0 | 1 | ||||||||
| 2 | 0 | 6 | 7 | 0 | 7* | 1 | 1 | 3 | ||||||||
| 3 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 1* | ||||||||
| Total number of explants: 104 | ||||||||||||||||
| . | Genotype and treatment . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of glands . | Control . | Control + BSA . | Control + Fgf10 . | Fgf10–/– + BSA . | Fgf10–/– + Fgf10 . | Gli3Xt-JIXt-J . | Gli3Xt-JIXt-J + BSA . | Gli3Xt-JIXt-J + Fgf10 . | ||||||||
| 0 | 5 | 4 | 27 | 2 | 10 | 1 | 0 | 6 | ||||||||
| 1 | 0 | 6 | 4 | 0 | 6 | 0 | 0 | 1 | ||||||||
| 2 | 0 | 6 | 7 | 0 | 7* | 1 | 1 | 3 | ||||||||
| 3 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 1* | ||||||||
| Total number of explants: 104 | ||||||||||||||||
Row 1 lists the genotypes of the flanks cultured with either no bead implanted, or with a bead soaked in BSA or rFGF10. Column 1 lists the total number of developing glands in these flanks as observed by Wnt10b or TOPGAL expression. The body of the table lists the number of flanks in which gland formation was analyzed
rFGF10 induced a supernumerary rudiment in ∼30% (7/23) of Fgf10–/– flanks and 9% (1/11) of Gli3Xt-JIXt-J flanks, but never in flanks of control embryos
Abbreviations: n.d., not determined; rFGF10, recombinant FGF10