Table 1.

Summary

FigureInjectionTreatmentObservationTotal numberPositive
Fig. 1B,C  Empty vector None Ectopic ureteric bud 8 0 
Fig. 1B,C  Gfnf cDNA None Ectopic ureteric bud 8 8 
Fig. 2A, parts d,e Gfp vector None Increased branching 12 0 
Fig. 2A, parts d,e Wt1 None Increased branching 12 11 
Fig. 2B, parts a,b Gfp vector None Increased Vegfa expression 12 0 
Fig. 2B, parts a,b Wt1−/− None Increased Vegfa expression 20 19 
Not shown * Wt1−/+ None Increased Vegfa expression 6 6 
Not shown * Wt1+/− None Increased Vegfa expression 3 3 
Not shown * Wt1+/+ None Increased Vegfa expression 3 3 
Fig. 3B  None PBS buffer Increased branching and nephrons 4 0 
Fig. 3B  None Vegfa protein Increased branching and nephrons 4 4 
Not shown Gfp vector None Increased branching 10 0 
Not shown Vegfa cDNA None Increased branching 10 10 
Fig. 3C  None Goat IgG Decreased branching and nephrons 4 0 
Fig. 3C  None Vegfa Ab Decreased branching and nephrons 4 4 
Fig. 4B  None Rat-IgG Decreased branching and nephrons 8 0 
Fig. 4B  None Flk1 Ab Decreased branching and nephrons 8 8 
Not shown * Gfp vector None Decreased branching 5 0 
Not shown * dnFlk1 cDNA † None Decreased branching 5 5 
Fig. 5A  None Rat-IgG Decreased Pax2 expression 6 0 
B None Flk1 Ab Decreased Pax2 expression 6 5 
C None Rat-IgG Decreased Osrl expression 3 0 
D None Flk1 Ab Decreased Osrl expression 3 0 
E None Rat-IgG Decreased Nanog expression 3 0 
F None Flk1 Ab Decreased Nanog expression 3 3 
G None Rat-IgG Decreased Wnt4 expression 3 0 
H None Flk1 Ab Decreased Wnt4 expression 3 3 
I None Rat-IgG Decreased Wt1 expression 3 0 
J None Flk1 Ab Decreased Wt1 expression 3 0 
Fig. 6A, part a Gfp None Increased Pax2 expression 8 0 
A, part b Vegfa None Increased Pax2 expression 11 10 
Fig. 6A, parts d,e Gfp vector Rat-IgG (as control) Decreased Pax2 expression 6 0 
Fig. 6A, parts d,e Vegfa cDNA Rat-IgG (as control) Decreased Pax2 expression 6 0 
Fig. 6A, parts d,e Gfp vector Flk1 Ab Decreased Pax2 expression 6 6 
Fig. 6A, parts d,e Vegfa cDNA Flk1 Ab Decreased Pax2 expression 6 6 
Not shown Gfp vector DMSO (as control) Decreased Pax2 expression 6 0 
Not shown Vegfa cDNA DMSO (as control) Decreased Pax2 expression 6 0 
Not shown Gfp vector SU1498 Decreased Pax2 expression 6 6 
Not shown Vegfa cDNA SU1498 Decreased Pax2 expression 6 6 
Fig. 6B  Empty vector Rat-IgG (as control) Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA Rat-IgG (as control) Pax2 stimulation of Gdnf expression 3 3 
Fig. 6B  Empty vector Flk1 Ab Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA Flk1 Ab Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Empty vector DMSO (as control) Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA DMSO (as control) Pax2 stimulation of Gdnf expression 3 3 
Fig. 6B  Empty vector SU1498 Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA SU1498 Pax2 stimulation of Gdnf expression 3 0 
Fig. 6D, parts a-d Pax2 cDNA Rat-IgG Increased Pax2 mRNA expression 3 3 
Fig. 6D, parts a-d Pax2 cDNA Flk1 Ab Increased Pax2 mRNA expression 3 0 
Fig. 6D, parts a-d Pax2 cDNA DMSO Increased Pax2 mRNA expression 3 3 
Fig. 6D, parts a-d Pax2 cDNA SU1498 Increased Pax2 mRNA expression 3 0 
Fig. 6D, parts e-h Pax2 cDNA Rat-IgG Increased Pax2 protein expression 3 3 
Fig. 6D, parts e-h Pax2 cDNA Flk1 Ab Increased Pax2 protein expression 3 0 
Fig. 6D, parts e-h Pax2 cDNA DMSO Increased Pax2 protein expression 3 3 
Fig. 6D, parts e-h Pax2 cDNA SU1498 Increased Pax2 protein expression 3 0 
Fig. 6D, parts i,j Gfp vector Rat-IgG GFP fluorescence 3 3 
Fig. 6D, parts i,j Gfp vector Flk1 Ab GFP fluorescence 3 3 
Fig. 6D, parts i,j Gfp vector DMSO GFP fluorescence 3 3 
Fig. 6D, parts i,j Gfp vector SU1498 GFP fluorescence 3 3 
Fig. 7A  None PBS/Flk1 Ab Increased branching and nephrons 4 0 
Fig. 7A  None Gdnf/Flk1 Ab Increased branching and nephrons 4 4 
Fig. 7B  None PBS/Flk1 Ab Increased Pax2 mRNA expression 4 0 
Fig. 7B  None Gdnf/Flk1 Ab Increased Pax2 mRNA expression 4 4 
Fig. 7C  None Rat-IgG(48/48) Decreased branching and nephrons 4 0 
Fig. 7C  None Flk1 Ab(48/48) Decreased branching and nephrons 4 0 
FigureInjectionTreatmentObservationTotal numberPositive
Fig. 1B,C  Empty vector None Ectopic ureteric bud 8 0 
Fig. 1B,C  Gfnf cDNA None Ectopic ureteric bud 8 8 
Fig. 2A, parts d,e Gfp vector None Increased branching 12 0 
Fig. 2A, parts d,e Wt1 None Increased branching 12 11 
Fig. 2B, parts a,b Gfp vector None Increased Vegfa expression 12 0 
Fig. 2B, parts a,b Wt1−/− None Increased Vegfa expression 20 19 
Not shown * Wt1−/+ None Increased Vegfa expression 6 6 
Not shown * Wt1+/− None Increased Vegfa expression 3 3 
Not shown * Wt1+/+ None Increased Vegfa expression 3 3 
Fig. 3B  None PBS buffer Increased branching and nephrons 4 0 
Fig. 3B  None Vegfa protein Increased branching and nephrons 4 4 
Not shown Gfp vector None Increased branching 10 0 
Not shown Vegfa cDNA None Increased branching 10 10 
Fig. 3C  None Goat IgG Decreased branching and nephrons 4 0 
Fig. 3C  None Vegfa Ab Decreased branching and nephrons 4 4 
Fig. 4B  None Rat-IgG Decreased branching and nephrons 8 0 
Fig. 4B  None Flk1 Ab Decreased branching and nephrons 8 8 
Not shown * Gfp vector None Decreased branching 5 0 
Not shown * dnFlk1 cDNA † None Decreased branching 5 5 
Fig. 5A  None Rat-IgG Decreased Pax2 expression 6 0 
B None Flk1 Ab Decreased Pax2 expression 6 5 
C None Rat-IgG Decreased Osrl expression 3 0 
D None Flk1 Ab Decreased Osrl expression 3 0 
E None Rat-IgG Decreased Nanog expression 3 0 
F None Flk1 Ab Decreased Nanog expression 3 3 
G None Rat-IgG Decreased Wnt4 expression 3 0 
H None Flk1 Ab Decreased Wnt4 expression 3 3 
I None Rat-IgG Decreased Wt1 expression 3 0 
J None Flk1 Ab Decreased Wt1 expression 3 0 
Fig. 6A, part a Gfp None Increased Pax2 expression 8 0 
A, part b Vegfa None Increased Pax2 expression 11 10 
Fig. 6A, parts d,e Gfp vector Rat-IgG (as control) Decreased Pax2 expression 6 0 
Fig. 6A, parts d,e Vegfa cDNA Rat-IgG (as control) Decreased Pax2 expression 6 0 
Fig. 6A, parts d,e Gfp vector Flk1 Ab Decreased Pax2 expression 6 6 
Fig. 6A, parts d,e Vegfa cDNA Flk1 Ab Decreased Pax2 expression 6 6 
Not shown Gfp vector DMSO (as control) Decreased Pax2 expression 6 0 
Not shown Vegfa cDNA DMSO (as control) Decreased Pax2 expression 6 0 
Not shown Gfp vector SU1498 Decreased Pax2 expression 6 6 
Not shown Vegfa cDNA SU1498 Decreased Pax2 expression 6 6 
Fig. 6B  Empty vector Rat-IgG (as control) Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA Rat-IgG (as control) Pax2 stimulation of Gdnf expression 3 3 
Fig. 6B  Empty vector Flk1 Ab Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA Flk1 Ab Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Empty vector DMSO (as control) Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA DMSO (as control) Pax2 stimulation of Gdnf expression 3 3 
Fig. 6B  Empty vector SU1498 Pax2 stimulation of Gdnf expression 3 0 
Fig. 6B  Pax2 cDNA SU1498 Pax2 stimulation of Gdnf expression 3 0 
Fig. 6D, parts a-d Pax2 cDNA Rat-IgG Increased Pax2 mRNA expression 3 3 
Fig. 6D, parts a-d Pax2 cDNA Flk1 Ab Increased Pax2 mRNA expression 3 0 
Fig. 6D, parts a-d Pax2 cDNA DMSO Increased Pax2 mRNA expression 3 3 
Fig. 6D, parts a-d Pax2 cDNA SU1498 Increased Pax2 mRNA expression 3 0 
Fig. 6D, parts e-h Pax2 cDNA Rat-IgG Increased Pax2 protein expression 3 3 
Fig. 6D, parts e-h Pax2 cDNA Flk1 Ab Increased Pax2 protein expression 3 0 
Fig. 6D, parts e-h Pax2 cDNA DMSO Increased Pax2 protein expression 3 3 
Fig. 6D, parts e-h Pax2 cDNA SU1498 Increased Pax2 protein expression 3 0 
Fig. 6D, parts i,j Gfp vector Rat-IgG GFP fluorescence 3 3 
Fig. 6D, parts i,j Gfp vector Flk1 Ab GFP fluorescence 3 3 
Fig. 6D, parts i,j Gfp vector DMSO GFP fluorescence 3 3 
Fig. 6D, parts i,j Gfp vector SU1498 GFP fluorescence 3 3 
Fig. 7A  None PBS/Flk1 Ab Increased branching and nephrons 4 0 
Fig. 7A  None Gdnf/Flk1 Ab Increased branching and nephrons 4 4 
Fig. 7B  None PBS/Flk1 Ab Increased Pax2 mRNA expression 4 0 
Fig. 7B  None Gdnf/Flk1 Ab Increased Pax2 mRNA expression 4 4 
Fig. 7C  None Rat-IgG(48/48) Decreased branching and nephrons 4 0 
Fig. 7C  None Flk1 Ab(48/48) Decreased branching and nephrons 4 0 

The number of times each experiment was performed and the outcomes discussed in the text and shown in each figure.

*

Data not shown in figures but referred to in the text.

†

Dominant negative Flk1 cDNA referred to in the text.

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