Sequence of primers for PCR
| Gene . | Sequence . | Product length (base pairs) . |
|---|---|---|
| β-Actin | F: 5′-AAGCAGGAGTACGATGAGTCTG-3′ | 278 |
| R: 5′-GGTAAACGCTTCTGGAATGAC-3′ | ||
| Elastase B | F: 5′-TTGCGGTGGAAGCCTTATTGACA-3′ | 487 |
| R: 5′-AGCGCAGTTTAGAGGACCACCAGA-3′ | ||
| foxA2 | F: 5′-CATCGCAAGCTCCAAATCT-3′ | 151 |
| R: 5′-TGCAGATCCAGATGGTGCAT-3′ | ||
| i-fabp | F: 5′-AAGTCGACCGCAATGAGAAC-3′ | 405 |
| R: 5′-GTTTGACATTGGGAGTGCAG-3′ | ||
| sox17 | F: 5′-ACCCGCTCAGTCCGCTCTCAG-3′ | 516 |
| R: 5′-ACTCAGTTGTCCCGGCATCCATAG-3′ | ||
| Transferrin | F: 5′-GAGAAAATCAAGCGCAAAGAAGC-3′ | 441 |
| R: 5′-AGCCCCATCATAGCCATAATACTG-3′ |
| Gene . | Sequence . | Product length (base pairs) . |
|---|---|---|
| β-Actin | F: 5′-AAGCAGGAGTACGATGAGTCTG-3′ | 278 |
| R: 5′-GGTAAACGCTTCTGGAATGAC-3′ | ||
| Elastase B | F: 5′-TTGCGGTGGAAGCCTTATTGACA-3′ | 487 |
| R: 5′-AGCGCAGTTTAGAGGACCACCAGA-3′ | ||
| foxA2 | F: 5′-CATCGCAAGCTCCAAATCT-3′ | 151 |
| R: 5′-TGCAGATCCAGATGGTGCAT-3′ | ||
| i-fabp | F: 5′-AAGTCGACCGCAATGAGAAC-3′ | 405 |
| R: 5′-GTTTGACATTGGGAGTGCAG-3′ | ||
| sox17 | F: 5′-ACCCGCTCAGTCCGCTCTCAG-3′ | 516 |
| R: 5′-ACTCAGTTGTCCCGGCATCCATAG-3′ | ||
| Transferrin | F: 5′-GAGAAAATCAAGCGCAAAGAAGC-3′ | 441 |
| R: 5′-AGCCCCATCATAGCCATAATACTG-3′ |
F, forward; R, reverse.