Construct . | Detectable GFP* . | Total embryos . | Relative GFPexpression† . |
---|---|---|---|
Esr1-FL | 57 (44%) | 128 | +++ |
Esr1 (RV) | 123 (56%) | 219 | +++ |
Esr1 (Hin3) | 78 (28%) | 277 | + |
Esr1 (RV) mS1S2 | 34 (31%) | 109 | - |
Esr1 (RV) mS1 | 7 (5%) | 142 | - |
Esr1 (RV) mS2 | 39 (53%) | 73 | +++ |
Esr1 (RV) mE1E2 | 19 (32%) | 59 | +++ |
Esr1 (RV) 3xmSuH | 10 (5%) | 210 | + |
Esr1 (RV) mE2a | 79 (55%) | 142 | +++ |
Esr1 (RV) mE2b | 120 (68%) | 176 | +++ |
Esr1 (RV) mE3 | 49 (90%) | 54 | +++ |
Esr1 (RV) mE123 | 110 (72%) | 152 | +++ |
Esr1 (RV) mS4 | 22 (18%) | 121 | + |
Esr10-FL | 140 (45%) | 310 | +++ |
Esr10 (Dra) | 146 (45%) | 325 | +++ |
Esr10 (Pst) | 138 (40%) | 344 | - |
Esr10-FL m1E1E2 | 15 (12%) | 122 | - |
Esr10 (Dra) m1E1E2 | 83 (40%) | 208 | - |
Esr10 (Dra) m2E1E2 | 12 (5%) | 226 | - |
Esr10 (Dra) mS1 | 0 | 214 | - |
Esr10 (Dra) mS2 | 13 (11%) | 120 | - |
Esr10 (Dra) mCAAT | 72 (52%) | 140 | +++ |
Esr7 | 28 (40%) | 70 | GFP+ |
Construct . | Detectable GFP* . | Total embryos . | Relative GFPexpression† . |
---|---|---|---|
Esr1-FL | 57 (44%) | 128 | +++ |
Esr1 (RV) | 123 (56%) | 219 | +++ |
Esr1 (Hin3) | 78 (28%) | 277 | + |
Esr1 (RV) mS1S2 | 34 (31%) | 109 | - |
Esr1 (RV) mS1 | 7 (5%) | 142 | - |
Esr1 (RV) mS2 | 39 (53%) | 73 | +++ |
Esr1 (RV) mE1E2 | 19 (32%) | 59 | +++ |
Esr1 (RV) 3xmSuH | 10 (5%) | 210 | + |
Esr1 (RV) mE2a | 79 (55%) | 142 | +++ |
Esr1 (RV) mE2b | 120 (68%) | 176 | +++ |
Esr1 (RV) mE3 | 49 (90%) | 54 | +++ |
Esr1 (RV) mE123 | 110 (72%) | 152 | +++ |
Esr1 (RV) mS4 | 22 (18%) | 121 | + |
Esr10-FL | 140 (45%) | 310 | +++ |
Esr10 (Dra) | 146 (45%) | 325 | +++ |
Esr10 (Pst) | 138 (40%) | 344 | - |
Esr10-FL m1E1E2 | 15 (12%) | 122 | - |
Esr10 (Dra) m1E1E2 | 83 (40%) | 208 | - |
Esr10 (Dra) m2E1E2 | 12 (5%) | 226 | - |
Esr10 (Dra) mS1 | 0 | 214 | - |
Esr10 (Dra) mS2 | 13 (11%) | 120 | - |
Esr10 (Dra) mCAAT | 72 (52%) | 140 | +++ |
Esr7 | 28 (40%) | 70 | GFP+ |
Constructs are described in the text and point mutant sequences are provided in Materials and methods. Total embryos are embryos completing gastrulation following sperm injection; embryos showing skin staining indicative of non-integrated DNA or severe morphological defects were not counted.
Scores do not reflect levels of tailbud, cranial ganglia and forebrain staining. Esr10 (Dra) m1E1E12 and m2E1E2 are different mutations in Esr10 E-boxes (see Materials and methods); m1E1E12 showed loss of neural staining but gave ectopic GFP in the heart field.
Any embryo showing GFP staining and therefore known to be transgenic but is not necessarily a measure of absolute transgenic efficiency.
Level of GFP staining in neural tube of stage 19-20 frog embryos relative to non-mutant controls included in every assay.