Activity of deletion and point mutants following Xngnr1injection
| Construct . | Induced by Xngnr1 . | Total embryos* . |
|---|---|---|
| Esr1 (RV) | 75 (78%) | 95 |
| Esr1 (Hin3) | 0 | 115 |
| Esr1 (RV) mS1 | 0 | 61 |
| Esr1 (RV) mE1E2 | 35 (83%) | 42 |
| Esr1 (RV) 3xmSuH | 0 | 72 |
| Esr10 (Dra) | 31 (88%) | 35 |
| Esr10 (Pst) | 0 | 100 |
| Esr10 (Dra) m1E1E2 | 3 (6%) | 47 |
| Esr10 (Dra) m2E1E2 | 4 (7%) | 54 |
| Esr10 (Dra) mS1 | 0 | 41 |
| Hairy2 (500 bp) | 0 | 250 |
| Construct . | Induced by Xngnr1 . | Total embryos* . |
|---|---|---|
| Esr1 (RV) | 75 (78%) | 95 |
| Esr1 (Hin3) | 0 | 115 |
| Esr1 (RV) mS1 | 0 | 61 |
| Esr1 (RV) mE1E2 | 35 (83%) | 42 |
| Esr1 (RV) 3xmSuH | 0 | 72 |
| Esr10 (Dra) | 31 (88%) | 35 |
| Esr10 (Pst) | 0 | 100 |
| Esr10 (Dra) m1E1E2 | 3 (6%) | 47 |
| Esr10 (Dra) m2E1E2 | 4 (7%) | 54 |
| Esr10 (Dra) mS1 | 0 | 41 |
| Hairy2 (500 bp) | 0 | 250 |
Constructs are described in the text. Hairy2 is identical to`H2pm' (Davis et al.,2001).
Total surviving embryos scored transgenic based on GFP signals and presence of β-gal (i.e. injected with Xngnr1 RNA). Except for wild-type Esr1 (RV), Esr10 (Dra) and H2A, this number probably greatly underestimates the number of transgenic embryos because (with the exception of the Esr1/RV construct mE1E2) deletion and point mutants show minimal GFP expression.