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Table 1.

Correlated pouch and cartilage phenotypes in fgf8-;fli1-GFP animals

Hyoid cartilage phenotype
Arch structure%(n)Wild typeHM reducedHM lossVentral lossShape changeEctopic
Wild type 74 59 33 
No p1 100 
Deformed p1 88 12 
Shifted p1 100 
Ectopic p1 50 50 
Arch shape 12 13 13 20 40 
Hyoid cartilage phenotype
Arch structure%(n)Wild typeHM reducedHM lossVentral lossShape changeEctopic
Wild type 74 59 33 
No p1 100 
Deformed p1 88 12 
Shifted p1 100 
Ectopic p1 50 50 
Arch shape 12 13 13 20 40 

Percentage of sides with each phenotype is listed for fgf8-;fli1-GFP animals (n=128). In 100 wild-type animals, none of these phenotypes were seen. At 33 hpf, individual sides of fgf8-; fli1-GFP mutant animals were scored under a fluorescence dissecting microscope for pharyngeal arch structure. Hyoid arches of mutant sides were scored as percentage of the total having the following morphology: wild type, missing the first pouch (no p1), deformed first pouch (deformed p1), shifted first pouch (shifted p1), ectopic first pouch in addition to normal first pouch (ectopic p1), and miscellaneous defects that affected arch shape but not first pouch structure (arch shape). Individuals were subsequently grown to 4 days to examine hyoid cartilage structure. Percentages of each arch structure category having the following hyoid cartilage phenotypes are shown: wild type, reduction of HM (HM reduced),loss of HM (HM loss), loss of ventral cartilage (ventral loss), rearrangement of the cartilage pattern (shape change), and ectopic cartilage elements(ectopic).

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