. | . | Hyoid cartilage phenotype . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Arch structure . | %(n) . | Wild type . | HM reduced . | HM loss . | Ventral loss . | Shape change . | Ectopic . | |||||
Wild type | 74 | 59 | 33 | 2 | 0 | 6 | 0 | |||||
No p1 | 2 | 0 | 100 | 0 | 0 | 0 | 0 | |||||
Deformed p1 | 6 | 0 | 0 | 88 | 0 | 12 | 0 | |||||
Shifted p1 | 2 | 0 | 0 | 0 | 0 | 100 | 0 | |||||
Ectopic p1 | 5 | 0 | 0 | 0 | 0 | 50 | 50 | |||||
Arch shape | 12 | 7 | 13 | 13 | 20 | 40 | 7 |
. | . | Hyoid cartilage phenotype . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Arch structure . | %(n) . | Wild type . | HM reduced . | HM loss . | Ventral loss . | Shape change . | Ectopic . | |||||
Wild type | 74 | 59 | 33 | 2 | 0 | 6 | 0 | |||||
No p1 | 2 | 0 | 100 | 0 | 0 | 0 | 0 | |||||
Deformed p1 | 6 | 0 | 0 | 88 | 0 | 12 | 0 | |||||
Shifted p1 | 2 | 0 | 0 | 0 | 0 | 100 | 0 | |||||
Ectopic p1 | 5 | 0 | 0 | 0 | 0 | 50 | 50 | |||||
Arch shape | 12 | 7 | 13 | 13 | 20 | 40 | 7 |
Percentage of sides with each phenotype is listed for fgf8-;fli1-GFP animals (n=128). In 100 wild-type animals, none of these phenotypes were seen. At 33 hpf, individual sides of fgf8-; fli1-GFP mutant animals were scored under a fluorescence dissecting microscope for pharyngeal arch structure. Hyoid arches of mutant sides were scored as percentage of the total having the following morphology: wild type, missing the first pouch (no p1), deformed first pouch (deformed p1), shifted first pouch (shifted p1), ectopic first pouch in addition to normal first pouch (ectopic p1), and miscellaneous defects that affected arch shape but not first pouch structure (arch shape). Individuals were subsequently grown to 4 days to examine hyoid cartilage structure. Percentages of each arch structure category having the following hyoid cartilage phenotypes are shown: wild type, reduction of HM (HM reduced),loss of HM (HM loss), loss of ventral cartilage (ventral loss), rearrangement of the cartilage pattern (shape change), and ectopic cartilage elements(ectopic).