Scoring of transgenic embryos after PCR machine heatshock at various stages
| HS stage (n) . | C5 . | C4 . | C3 . | C2 . | C1 . | C1+2° tail . | No effect . |
|---|---|---|---|---|---|---|---|
| 3 hpf (20) | 100% | 0% | 0% | 0% | 0% | 0% | 0% |
| Shield (22) | 0% | 0% | 41% | 0% | 59% | 18% | 0% |
| 70% (23) | 0% | 0% | 0% | 0% | 100% | 17% | 0% |
| Bud (21) | 0% | 0% | 0% | 0% | 100% | 14% | 0% |
| 3-somite (25) | 0% | 0% | 0% | 0% | 100% | 8% | 0% |
| 6-somite (24) | 0% | 0% | 0% | 0% | 54% | 4% | 46% |
| 10-somite (25) | 0% | 0% | 0% | 0% | 0% | 0% | 100% |
| HS stage (n) . | C5 . | C4 . | C3 . | C2 . | C1 . | C1+2° tail . | No effect . |
|---|---|---|---|---|---|---|---|
| 3 hpf (20) | 100% | 0% | 0% | 0% | 0% | 0% | 0% |
| Shield (22) | 0% | 0% | 41% | 0% | 59% | 18% | 0% |
| 70% (23) | 0% | 0% | 0% | 0% | 100% | 17% | 0% |
| Bud (21) | 0% | 0% | 0% | 0% | 100% | 14% | 0% |
| 3-somite (25) | 0% | 0% | 0% | 0% | 100% | 8% | 0% |
| 6-somite (24) | 0% | 0% | 0% | 0% | 54% | 4% | 46% |
| 10-somite (25) | 0% | 0% | 0% | 0% | 0% | 0% | 100% |