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Table 2.

Scoring of transgenic embryos after PCR machine heatshock at various stages

HS stage (n)C5C4C3C2C1C1+2° tailNo effect
3 hpf (20) 100% 0% 0% 0% 0% 0% 0% 
Shield (22) 0% 0% 41% 0% 59% 18% 0% 
70% (23) 0% 0% 0% 0% 100% 17% 0% 
Bud (21) 0% 0% 0% 0% 100% 14% 0% 
3-somite (25) 0% 0% 0% 0% 100% 8% 0% 
6-somite (24) 0% 0% 0% 0% 54% 4% 46% 
10-somite (25) 0% 0% 0% 0% 0% 0% 100% 
HS stage (n)C5C4C3C2C1C1+2° tailNo effect
3 hpf (20) 100% 0% 0% 0% 0% 0% 0% 
Shield (22) 0% 0% 41% 0% 59% 18% 0% 
70% (23) 0% 0% 0% 0% 100% 17% 0% 
Bud (21) 0% 0% 0% 0% 100% 14% 0% 
3-somite (25) 0% 0% 0% 0% 100% 8% 0% 
6-somite (24) 0% 0% 0% 0% 54% 4% 46% 
10-somite (25) 0% 0% 0% 0% 0% 0% 100% 
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