Skip to Main Content
Table 1.

Estimated osmotic contribution of initial osmolytes in the hemolymph and osmolytes produced during exposure of Belgica antarctica larvae to hyperosmotic seawater (∼1000 mOsm kg–1)

Seawater (1000 mOsm kg–1)
Control (freshwater) 6
Days of exposure0136
Osmolality (mOsm kg–1407±7a 491±10b 646±12c 779±15d 391±10a 
Total body water content (g H2O g–1 DM) 2.51±0.07a 2.17±0.05b 2.07±0.03b 1.89±0.05c 2.46±0.06a 
Osmotically active water (OAW)content* (g H2O g–1 DM) 2.04 1.72 1.63 1.46 1.99 
Loss of OAW (%) – 15.7 21.1 28.5 – 
Osmotic contribution of original solutes due to loss of OAW (mOsm kg–1– 480 516 569 – 
Osmolyte concentration (μg mg–1 DM)      
   Glycerol 2.17±0.27a 2.96±0.46a 6.87±0.58b 9.56±0.46c 1.94±0.31a 
   Glucose 3.75±0.34a 4.71±0.42a 8.30±0.48b 12.68±0.54c 3.82±0.28a 
   Trehalose 4.91±0.31a 6.62±0.36b 9.11±0.41c 14.38±0.53d 4.23±0.37a 
Osmotic contribution of synthesized osmolytes(mOsm) – 11 54 108 – 
Total explainable osmotic pressure (%) – ∼100 88.2 86.9 – 
Seawater (1000 mOsm kg–1)
Control (freshwater) 6
Days of exposure0136
Osmolality (mOsm kg–1407±7a 491±10b 646±12c 779±15d 391±10a 
Total body water content (g H2O g–1 DM) 2.51±0.07a 2.17±0.05b 2.07±0.03b 1.89±0.05c 2.46±0.06a 
Osmotically active water (OAW)content* (g H2O g–1 DM) 2.04 1.72 1.63 1.46 1.99 
Loss of OAW (%) – 15.7 21.1 28.5 – 
Osmotic contribution of original solutes due to loss of OAW (mOsm kg–1– 480 516 569 – 
Osmolyte concentration (μg mg–1 DM)      
   Glycerol 2.17±0.27a 2.96±0.46a 6.87±0.58b 9.56±0.46c 1.94±0.31a 
   Glucose 3.75±0.34a 4.71±0.42a 8.30±0.48b 12.68±0.54c 3.82±0.28a 
   Trehalose 4.91±0.31a 6.62±0.36b 9.11±0.41c 14.38±0.53d 4.23±0.37a 
Osmotic contribution of synthesized osmolytes(mOsm) – 11 54 108 – 
Total explainable osmotic pressure (%) – ∼100 88.2 86.9 – 

Values are mean ± s.e.m. Different letters indicate significant differences between days of exposure (ANOVA; Bonferroni-Dunn test)

*

OAW was calculated from Worland et al.(1998). [(OIW) = 0.069(TBW) +0.3, where OIW is osmotically inactive water content, TBW is total body water content and is the sum of OIW and OAW]

Assuming that osmolytes are only dissolved in OAW

Close Modal

or Create an Account

Close Modal
Close Modal