Information on the gene-specific primers used for qRT-PCR analysis of retinal quadrants from parr, 2 day T4-treated parr and natural smolt
| Gene . | Forward primer . | Reverse primer . | Amplicon size (bp) . | Primer concentration (μmol l–1) . | MgCl concentration (mmol l–1) . | Linear R2 value/reaction efficiency (%) . |
|---|---|---|---|---|---|---|
| SWS1 | GGCTTTCTACCTACAGACTGC | CCTGCTATTGAACCCATGC | 258 | 200 | 3 | 0.99/95.0 |
| TRα | GCACAACATTCCCCACTTCT | AGTTCGTTGGGACACTCCAC | 117 | 300 | 5 | 0.99/99.4 |
| TRβ | TCACCTGTGAAGGATGCAAG | GACAGCGATGCACTTCTTGA | 152 | 300 | 5 | 0.99/97.0 |
| D2 | ATTTTGTATGCCGATGCACA | TACGGCGCTAACCTCTGTTT | 207 | 300 | 5 | 0.98/92.4 |
| L8 | GGTGTGGCTATGAATCCTGT | ACGACGAGCAGCAATAAGAC | 113 | 200 | 3 | 0.99/100.6 |
| Gene . | Forward primer . | Reverse primer . | Amplicon size (bp) . | Primer concentration (μmol l–1) . | MgCl concentration (mmol l–1) . | Linear R2 value/reaction efficiency (%) . |
|---|---|---|---|---|---|---|
| SWS1 | GGCTTTCTACCTACAGACTGC | CCTGCTATTGAACCCATGC | 258 | 200 | 3 | 0.99/95.0 |
| TRα | GCACAACATTCCCCACTTCT | AGTTCGTTGGGACACTCCAC | 117 | 300 | 5 | 0.99/99.4 |
| TRβ | TCACCTGTGAAGGATGCAAG | GACAGCGATGCACTTCTTGA | 152 | 300 | 5 | 0.99/97.0 |
| D2 | ATTTTGTATGCCGATGCACA | TACGGCGCTAACCTCTGTTT | 207 | 300 | 5 | 0.98/92.4 |
| L8 | GGTGTGGCTATGAATCCTGT | ACGACGAGCAGCAATAAGAC | 113 | 200 | 3 | 0.99/100.6 |