Sequences of synthetic polynucleotides used as templates for generation of standard curves, primers and GenBank references used for mRNA quantification of α1a, α1b, α1c,α 3 and β1 subunit isoforms of the Salmo salar Na+,K+–ATPase
| Transcript . | cDNA sequence (5′-3′) used for quantification* . | Primers 5′-3′ . | Reference . |
|---|---|---|---|
| α1a | TTCCAAAGGCAAATGGGTTTAATATCATTGTAAAAATGAACGATTCATTACAA | F: CCCAGGATCACTCAATGTCAC | O. mykiss/ |
| GGCAATACTGCAGCAGAGTGACATTGAGTGATCCTGGGACGGCCTC | R: CCAAAGGCAAATGGGTTTAAT | AY319391 | |
| α1b | GCAGCTGAGTGCACCATCACAGTGTTCATTGGATGTAATGATAAGGTAGCAT | F: CTGCTACATCTCAACCAACAACATT | O. mykiss/ |
| ATTGAAATGTAGTGT AATGTTGTTGGTTGAGATGTAGCAG CAGAGTG | R: CACCATCACAGTGTTCATTGGAT | AY319390 | |
| α1c | CAGAACTTAAAATTCCGAG CAGCAAGACAACCATGCAAGAACTTGAGCGATG | F: GAGAGGGAGACGTACTACTAGAAAGCA | O. mykiss/ |
| CAAACGGGATGCTTTCTAGTAGTACGTCTCCCTCTCCACCCAACCTC | R: CAGCAAGACAACCATGCAAGA | AY319389 | |
| α3 | CTTGTGGGTATCAGGCTCAACT GGGACGACCGCGCTTGCAACGACCTGGA | F: TCCACGATCTTCCTCTGTTCA | S. salar/ |
| AGACAGCTATGGCCAGCAATGGACATA TGAACAGAGGAAGATCGTGGAG | R: TTGTGGGTATCAGGCTCAACT | CX355357 | |
| β1 | TTACCGGCGTCCTCCTCTCTCTTGTTGGTGCAAAAGATGGGCATGACGTTG | F: CCAATGAAAGCATCCCTAG | S. salar/ |
| GGCTGGACCTTGGTCTGCGCTCCCTCAGGGATGCTTTCATTGGAGCTG | R: GGCGTCCTCCTCTCTCTTGT | AJ250810 |
| Transcript . | cDNA sequence (5′-3′) used for quantification* . | Primers 5′-3′ . | Reference . |
|---|---|---|---|
| α1a | TTCCAAAGGCAAATGGGTTTAATATCATTGTAAAAATGAACGATTCATTACAA | F: CCCAGGATCACTCAATGTCAC | O. mykiss/ |
| GGCAATACTGCAGCAGAGTGACATTGAGTGATCCTGGGACGGCCTC | R: CCAAAGGCAAATGGGTTTAAT | AY319391 | |
| α1b | GCAGCTGAGTGCACCATCACAGTGTTCATTGGATGTAATGATAAGGTAGCAT | F: CTGCTACATCTCAACCAACAACATT | O. mykiss/ |
| ATTGAAATGTAGTGT AATGTTGTTGGTTGAGATGTAGCAG CAGAGTG | R: CACCATCACAGTGTTCATTGGAT | AY319390 | |
| α1c | CAGAACTTAAAATTCCGAG CAGCAAGACAACCATGCAAGAACTTGAGCGATG | F: GAGAGGGAGACGTACTACTAGAAAGCA | O. mykiss/ |
| CAAACGGGATGCTTTCTAGTAGTACGTCTCCCTCTCCACCCAACCTC | R: CAGCAAGACAACCATGCAAGA | AY319389 | |
| α3 | CTTGTGGGTATCAGGCTCAACT GGGACGACCGCGCTTGCAACGACCTGGA | F: TCCACGATCTTCCTCTGTTCA | S. salar/ |
| AGACAGCTATGGCCAGCAATGGACATA TGAACAGAGGAAGATCGTGGAG | R: TTGTGGGTATCAGGCTCAACT | CX355357 | |
| β1 | TTACCGGCGTCCTCCTCTCTCTTGTTGGTGCAAAAGATGGGCATGACGTTG | F: CCAATGAAAGCATCCCTAG | S. salar/ |
| GGCTGGACCTTGGTCTGCGCTCCCTCAGGGATGCTTTCATTGGAGCTG | R: GGCGTCCTCCTCTCTCTTGT | AJ250810 |
The sequence of each synthetic polynucleotides was based on the corresponding reverse strand, anti-sense gene sequence listed as reference. *Shaded areas of the polynucleotide sequences indicate primer regions