The Company of Biologists

Table 1.

Primers for quantitative RT-PCR

Gene	Forward (5′–3′)	Reverse (5′–3′)	Fragment (bp)
CD14	ATTGCGATGTGCATTTCCG	GAAAGTCCACTGCTTTGCTCG	109
TLR4	TTATGTGGAGGGGAAAAGCAC	GGAGGCAGATTGGAATGAG	124
TNF-α	GGACATCACCAAACGACACC	CCCCAGCAAGAAGAAGGAAG	184
GAPDH	GGTAAGCTGTGGAGTGATGGGCG	TGGGAGTAGGAACACGGAAGGCC	118
23S rRNA	CGATGTCGGCTCTTCCTATC	TCCTCAGCCAAGCACATACA	205
β-Actin	GCGTCACCCACAACGTACCCATCT	GATGTCACGCACAATTTCCCGCTC	140

Gene	Forward (5′–3′)	Reverse (5′–3′)	Fragment (bp)
CD14	ATTGCGATGTGCATTTCCG	GAAAGTCCACTGCTTTGCTCG	109
TLR4	TTATGTGGAGGGGAAAAGCAC	GGAGGCAGATTGGAATGAG	124
TNF-α	GGACATCACCAAACGACACC	CCCCAGCAAGAAGAAGGAAG	184
GAPDH	GGTAAGCTGTGGAGTGATGGGCG	TGGGAGTAGGAACACGGAAGGCC	118
23S rRNA	CGATGTCGGCTCTTCCTATC	TCCTCAGCCAAGCACATACA	205
β-Actin	GCGTCACCCACAACGTACCCATCT	GATGTCACGCACAATTTCCCGCTC	140

Primers used for quantitative PCR of CD14 and TLR4 expression in the organs of the pouch young. bp, base pairs. Primers were manufactured by Sigma-Genosys(Castle Hill, NSW, Australia)