Gene . | Forward (5′–3′) . | Reverse (5′–3′) . | Fragment (bp) . |
---|---|---|---|
CD14 | ATTGCGATGTGCATTTCCG | GAAAGTCCACTGCTTTGCTCG | 109 |
TLR4 | TTATGTGGAGGGGAAAAGCAC | GGAGGCAGATTGGAATGAG | 124 |
TNF-α | GGACATCACCAAACGACACC | CCCCAGCAAGAAGAAGGAAG | 184 |
GAPDH | GGTAAGCTGTGGAGTGATGGGCG | TGGGAGTAGGAACACGGAAGGCC | 118 |
23S rRNA | CGATGTCGGCTCTTCCTATC | TCCTCAGCCAAGCACATACA | 205 |
β-Actin | GCGTCACCCACAACGTACCCATCT | GATGTCACGCACAATTTCCCGCTC | 140 |
Gene . | Forward (5′–3′) . | Reverse (5′–3′) . | Fragment (bp) . |
---|---|---|---|
CD14 | ATTGCGATGTGCATTTCCG | GAAAGTCCACTGCTTTGCTCG | 109 |
TLR4 | TTATGTGGAGGGGAAAAGCAC | GGAGGCAGATTGGAATGAG | 124 |
TNF-α | GGACATCACCAAACGACACC | CCCCAGCAAGAAGAAGGAAG | 184 |
GAPDH | GGTAAGCTGTGGAGTGATGGGCG | TGGGAGTAGGAACACGGAAGGCC | 118 |
23S rRNA | CGATGTCGGCTCTTCCTATC | TCCTCAGCCAAGCACATACA | 205 |
β-Actin | GCGTCACCCACAACGTACCCATCT | GATGTCACGCACAATTTCCCGCTC | 140 |
Primers used for quantitative PCR of CD14 and TLR4 expression in the organs of the pouch young. bp, base pairs. Primers were manufactured by Sigma-Genosys(Castle Hill, NSW, Australia)