. | . | . | Probe . | . | . | . | . | . | |||
---|---|---|---|---|---|---|---|---|---|---|---|
Species . | . | . | Volume given (μl) . | d-[14C]glucose (Bq) . | [14C]3-OMG (Bq) . | l-[3H]glucose (Bq) . | NaCl (mmol l–1) . | Unlabeled d-glucose (mmol l–1) . | |||
RL | i.m. | 125 | 4.4×105 | 1.1×106 | 175 | ||||||
Oral | 300 | 8.9×105 | 2.1×106 | 75 | 200 | ||||||
Oral | Probe distribution in body tissues | 300 | 6.7×105 | 6.7×106 | 75 | 200 | |||||
RWB | i.m. | Trial A | 125 | 3.7×105 | 3.5×105 | 8.3×105 | 175 | ||||
i.m. | Trial B | 125 | 8.3×105 | 175 | |||||||
Oral | Trial A | 300 | 7.4×105 | 1.7×105 | 75 | 200 | |||||
Oral | Trial B | 300 | 7×105 | 1.7×105 | 75 | 200 | |||||
Oral | Probe distribution in body tissues | 300 | 6.7×105 | 6.7×105 | 75 | 200 |
. | . | . | Probe . | . | . | . | . | . | |||
---|---|---|---|---|---|---|---|---|---|---|---|
Species . | . | . | Volume given (μl) . | d-[14C]glucose (Bq) . | [14C]3-OMG (Bq) . | l-[3H]glucose (Bq) . | NaCl (mmol l–1) . | Unlabeled d-glucose (mmol l–1) . | |||
RL | i.m. | 125 | 4.4×105 | 1.1×106 | 175 | ||||||
Oral | 300 | 8.9×105 | 2.1×106 | 75 | 200 | ||||||
Oral | Probe distribution in body tissues | 300 | 6.7×105 | 6.7×106 | 75 | 200 | |||||
RWB | i.m. | Trial A | 125 | 3.7×105 | 3.5×105 | 8.3×105 | 175 | ||||
i.m. | Trial B | 125 | 8.3×105 | 175 | |||||||
Oral | Trial A | 300 | 7.4×105 | 1.7×105 | 75 | 200 | |||||
Oral | Trial B | 300 | 7×105 | 1.7×105 | 75 | 200 | |||||
Oral | Probe distribution in body tissues | 300 | 6.7×105 | 6.7×105 | 75 | 200 |
RL, rainbow lorikeet; RWB, red wattlebird; i.m. intramuscular injection. Oral solutions were made up to conditions relatively saturating for the mediated glucose transporters (Chang et al., 2004). The total osmolality of the oral and injection solutions was controlled at approximately 350 mmol kg–1, so that the solutions were isosmotic with avian blood(Goldstein and Skadhauge,2000). Differences in the amounts of radioactivity in the probe solutions reflect differences in body mass between these two species