Table 1.

Primers used for the experiments

Primer nameSequence (5′–3′)Usage
HO1-F1 CCCACACCTAACGCAAACTGG Gene cloning 
HO1-R1 GCTGGATGAGGCAACCAGAGC RT-PCR 
HO1E-F2 CCGCTCGAGCGATGGAATCCACGAAAA Plasmid construction 
HO1E-R2 TCCCCGCGGGAAAAGCATAAACTCCCATT Plasmid construction 
HO1D-F3 CCGCTCGAGATGGAATCCACGAA Plasmid construction 
HO1D-R3 GGGGTACCAAAAGCATAAACTCCC Plasmid construction 
HO1-F4 GGAAGAGAGTAACAGTGCC Real-time PCR 
HO1-R4 AGCACTTCCTGTCTCTGCTG RT-PCR 
SMART-F AACGCAGAGTACGCGGG RACE-PCR 
SMART-R CAGAGTACT16 RACE 
β-Actin F CACTGTGCCCATCTACGAG Real-time PCR 
β-Actin R CCATCTCCTGCTCGAAGTC RT-PCR 
T7 TAATACGACTCACTATAGGG RT-PCR 
Primer nameSequence (5′–3′)Usage
HO1-F1 CCCACACCTAACGCAAACTGG Gene cloning 
HO1-R1 GCTGGATGAGGCAACCAGAGC RT-PCR 
HO1E-F2 CCGCTCGAGCGATGGAATCCACGAAAA Plasmid construction 
HO1E-R2 TCCCCGCGGGAAAAGCATAAACTCCCATT Plasmid construction 
HO1D-F3 CCGCTCGAGATGGAATCCACGAA Plasmid construction 
HO1D-R3 GGGGTACCAAAAGCATAAACTCCC Plasmid construction 
HO1-F4 GGAAGAGAGTAACAGTGCC Real-time PCR 
HO1-R4 AGCACTTCCTGTCTCTGCTG RT-PCR 
SMART-F AACGCAGAGTACGCGGG RACE-PCR 
SMART-R CAGAGTACT16 RACE 
β-Actin F CACTGTGCCCATCTACGAG Real-time PCR 
β-Actin R CCATCTCCTGCTCGAAGTC RT-PCR 
T7 TAATACGACTCACTATAGGG RT-PCR 
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