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Table 1.

Primers used for quantitative real-time PCR

Tilapia geneGenBank accession no.Sense (5′ to 3′)Antisense (5′ to 3′)Product size (bp)
NKCC1a AY513737 GGAGGCAAGATCAACAGGATTG AATGTCCGAAAAGTCTATCCTGAACT 84 
NKCC1b AY513738 AGAACTTTGGTCCAGAATTCAGAGAT CCAGTAGCTGCAGGGAAGAAA 76 
NKCC2 AY513739 CTCATCGGTGACCTCAACAC CAACCTGGAGATTTGGCATA 113 
NCC EU518934 CCGAAAGGCACCCTAATGG CTACACTTGCACCAGAAGTGACAA 79 
NHE3 AB326212 AAGCGGCACCCATCACTACA GAGCCAGCAAACCAGAATCCA 98 
H+-ATPase AB369668 CCACAGCTCAGAGCGACAACA GCATACTCGGCCTTGATCTTG 136 
18S rRNA AF497908 CGGAGAGGGAGCCTGAGAA AGTCGGGAGTGGGTAATTTGC 73 
Tilapia geneGenBank accession no.Sense (5′ to 3′)Antisense (5′ to 3′)Product size (bp)
NKCC1a AY513737 GGAGGCAAGATCAACAGGATTG AATGTCCGAAAAGTCTATCCTGAACT 84 
NKCC1b AY513738 AGAACTTTGGTCCAGAATTCAGAGAT CCAGTAGCTGCAGGGAAGAAA 76 
NKCC2 AY513739 CTCATCGGTGACCTCAACAC CAACCTGGAGATTTGGCATA 113 
NCC EU518934 CCGAAAGGCACCCTAATGG CTACACTTGCACCAGAAGTGACAA 79 
NHE3 AB326212 AAGCGGCACCCATCACTACA GAGCCAGCAAACCAGAATCCA 98 
H+-ATPase AB369668 CCACAGCTCAGAGCGACAACA GCATACTCGGCCTTGATCTTG 136 
18S rRNA AF497908 CGGAGAGGGAGCCTGAGAA AGTCGGGAGTGGGTAATTTGC 73 
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