Distributions of the numbers of MLH1 foci per bivalent
| . | . | . | Percentage of bivalents with: . | . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome . | Genotype (number of bivalents) . | Observed or expected* . | 0 foci . | 1 focus . | 2 foci . | 3 foci . | 4 foci . | Observed vs expected P† . | Obs. wt vs obs. –/– P‡ . | ||||
| 1 | Wild type (112) | Observed | 0.0 | 24.1 | 67.0 | 8.9 | 0.0 | 0.31 | 0.15 | ||||
| Exp. (ν=8)§ | 0.4 | 26.9 | 60.3 | 12.2 | 0.2 | ||||||||
| Sycp3–/– (44) | Observed | 4.6 | 22.7 | 59.1 | 13.6 | 0.0 | 0.98 | ||||||
| Exp. (ν=10)§ | 0.3 | 27.2 | 63.0 | 9.5 | 0.1 | ||||||||
| 2 | Wild type (110) | Observed | 1.8 | 20.9 | 74.6 | 2.7 | 0.0 | 0.01 | 0.40 | ||||
| Exp. (ν=10)§ | 0.3 | 29.8 | 61.6 | 8.3 | 0.0 | ||||||||
| Sycp3–/– (47) | Observed | 2.1 | 21.3 | 68.1 | 8.5 | 0.0 | 0.36 | ||||||
| Exp. (ν=7)§ | 0.8 | 28.5 | 58.0 | 12.5 | 0.3 | ||||||||
| 18 | Wild type (105) | Observed | 2.9 | 89.5 | 7.6 | 0.0 | 0.0 | 0.91 | |||||
| Exp. (ν=1)¶ | 35.0 | 37.0 | 19.4 | 6.8 | 2.8 | 4.7×10–27 | |||||||
| Exp. (ν=17)** | 7.2 | 80.5 | 12.2 | 0.0 | 0.0 | 0.06 | |||||||
| Sycp3–/– (49) | Observed | 4.1 | 87.8 | 8.2 | 0.0 | 0.0 | |||||||
| Exp. (ν=1)¶ | 35.5 | 36.9 | 19.3 | 6.6 | 1.7 | 1.4×10–12 | |||||||
| Exp. (ν=8)** | 11.6 | 73.0 | 15.3 | 0.1 | 0.0 | 0.06 | |||||||
| 19 | Wild type (113) | Observed | 2.7 | 94.7 | 2.7 | 0.0 | 0.0 | 0.0004 | |||||
| Exp. (ν=1)¶ | 37.0 | 36.9 | 18.4 | 6.1 | 1.5 | 1.3×10–12 | |||||||
| Exp. (ν=33)** | 6.1 | 87.8 | 6.1 | 0.0 | 0.0 | 0.08 | |||||||
| Sycp3–/– (28) | Observed | 25.0 | 71.4 | 3.6 | 0.0 | 0.0 | |||||||
| Exp. (ν=1)¶ | 46.6 | 35.8 | 14.1 | 3.7 | 0.7 | 0.0004 | |||||||
| Exp. (ν=2)** | 36.7 | 49.1 | 12.8 | 1.3 | 0.07 | 0.2 | |||||||
| . | . | . | Percentage of bivalents with: . | . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome . | Genotype (number of bivalents) . | Observed or expected* . | 0 foci . | 1 focus . | 2 foci . | 3 foci . | 4 foci . | Observed vs expected P† . | Obs. wt vs obs. –/– P‡ . | ||||
| 1 | Wild type (112) | Observed | 0.0 | 24.1 | 67.0 | 8.9 | 0.0 | 0.31 | 0.15 | ||||
| Exp. (ν=8)§ | 0.4 | 26.9 | 60.3 | 12.2 | 0.2 | ||||||||
| Sycp3–/– (44) | Observed | 4.6 | 22.7 | 59.1 | 13.6 | 0.0 | 0.98 | ||||||
| Exp. (ν=10)§ | 0.3 | 27.2 | 63.0 | 9.5 | 0.1 | ||||||||
| 2 | Wild type (110) | Observed | 1.8 | 20.9 | 74.6 | 2.7 | 0.0 | 0.01 | 0.40 | ||||
| Exp. (ν=10)§ | 0.3 | 29.8 | 61.6 | 8.3 | 0.0 | ||||||||
| Sycp3–/– (47) | Observed | 2.1 | 21.3 | 68.1 | 8.5 | 0.0 | 0.36 | ||||||
| Exp. (ν=7)§ | 0.8 | 28.5 | 58.0 | 12.5 | 0.3 | ||||||||
| 18 | Wild type (105) | Observed | 2.9 | 89.5 | 7.6 | 0.0 | 0.0 | 0.91 | |||||
| Exp. (ν=1)¶ | 35.0 | 37.0 | 19.4 | 6.8 | 2.8 | 4.7×10–27 | |||||||
| Exp. (ν=17)** | 7.2 | 80.5 | 12.2 | 0.0 | 0.0 | 0.06 | |||||||
| Sycp3–/– (49) | Observed | 4.1 | 87.8 | 8.2 | 0.0 | 0.0 | |||||||
| Exp. (ν=1)¶ | 35.5 | 36.9 | 19.3 | 6.6 | 1.7 | 1.4×10–12 | |||||||
| Exp. (ν=8)** | 11.6 | 73.0 | 15.3 | 0.1 | 0.0 | 0.06 | |||||||
| 19 | Wild type (113) | Observed | 2.7 | 94.7 | 2.7 | 0.0 | 0.0 | 0.0004 | |||||
| Exp. (ν=1)¶ | 37.0 | 36.9 | 18.4 | 6.1 | 1.5 | 1.3×10–12 | |||||||
| Exp. (ν=33)** | 6.1 | 87.8 | 6.1 | 0.0 | 0.0 | 0.08 | |||||||
| Sycp3–/– (28) | Observed | 25.0 | 71.4 | 3.6 | 0.0 | 0.0 | |||||||
| Exp. (ν=1)¶ | 46.6 | 35.8 | 14.1 | 3.7 | 0.7 | 0.0004 | |||||||
| Exp. (ν=2)** | 36.7 | 49.1 | 12.8 | 1.3 | 0.07 | 0.2 | |||||||
For each chromosome, the expected percentages of bivalents with 0, 1, 2, 3 or 4 foci are based on the observed average number of MLH1 foci on that chromosome (Table 1), and the corrected estimate of the interference parameter ν (Table 2) for MLH1 foci on that chromosome. We determined the expected percentages by simulating the positions of MLH1 foci along at least 5000 bivalents for the corrected ν, rounded to an integer value, assuming that MLH1 foci are uniformly distributed along the bivalent (Fig. 1)
The P values in this column represent for each bivalent the probability of finding an as-bad or worse fit of the observed to the expected numbers due to sampling error (χ2 test; groups were combined if the expected numbers equaled 5 or less)
The P values in this column represent for each bivalent the probability of finding an as-large or larger difference between the observed values for the wild type and those for Sycp3–/– due to sampling error (Fisher's exact test)
Expected percentages of bivalents with 0, 1, 2, 3 or 4 foci for the corrected estimate of the interference parameter ν
Expected percentages of bivalents with 0, 1, 2, 3 or 4 foci if there were no interference between MLH1 foci (ν=1)
Lowest integer value of ν for which the expected distribution of the numbers of MLH1 foci per bivalent fits to the observed distribution (i.e. P>0.05)