Plasmids . | Description . | Source . |
---|---|---|
pCR2.1-TOPO | TA-cloning vector (for cloning of PCR fragments) | Invitrogen, NV Leek, The Netherlands |
pDC1 | A. nidulans argB selectable marker plasmid | Aramayo et al., 1989 |
pDM2 | mRFP1 with NotI in pCR2.1-Topo | This work |
pDM5 | gpd(p)::apsB::GFP in pBluescript KS— | This work |
pDM6 | KpnI-AscI fragment of mRFP1 cloned into pCR2.1-Topo | This work |
pDM8 | GFP replaced by mRFP1 in pMCB17apx-apsB | This work |
pJH19 | gpd(p)::stuA(NLS)::DsRedT4 and argB as selectable marker in pBluescript KS— | Toews et al., 2004 |
pJW18 | alcA(p)::stuA(NLS)::DsRedT4 and argB as selectable marker in pBluescript KS— | Toews et al., 2004 |
pMCB17 | GFP gene (pyr4::alcA(p)::gfp2-5) in pUC19 | Fernandez-Abalos et al., 1998 |
pMCB17apx | pMCB17 version for fusion of GFP to N-termini of proteins of interest | V. P. Efimov, Piscataway, USA |
p17apx-apsB | 1.5 kb of apsB in PacI/AscI restriction site of pMCB17apx | V. P. Efimov, Piscataway, USA |
pRF280 | gpd(p)::stuA(NLS)::GFP; argB in pRS31 | R. Fischer, Marburg, Germany |
pRS31 | gpd(p)::stuA(NLS)::GFP in pBluescript KS— | Suelmann et al., 1997 |
pRS48 | apsB tagged with GFP in EcoRV in pBluescript KS— | Suelmann et al., 1997 |
pRS50 | alcA(p)::apsB::GFP pBluescript KS— | R. Suelman, Marburg, Germany |
Plasmids . | Description . | Source . |
---|---|---|
pCR2.1-TOPO | TA-cloning vector (for cloning of PCR fragments) | Invitrogen, NV Leek, The Netherlands |
pDC1 | A. nidulans argB selectable marker plasmid | Aramayo et al., 1989 |
pDM2 | mRFP1 with NotI in pCR2.1-Topo | This work |
pDM5 | gpd(p)::apsB::GFP in pBluescript KS— | This work |
pDM6 | KpnI-AscI fragment of mRFP1 cloned into pCR2.1-Topo | This work |
pDM8 | GFP replaced by mRFP1 in pMCB17apx-apsB | This work |
pJH19 | gpd(p)::stuA(NLS)::DsRedT4 and argB as selectable marker in pBluescript KS— | Toews et al., 2004 |
pJW18 | alcA(p)::stuA(NLS)::DsRedT4 and argB as selectable marker in pBluescript KS— | Toews et al., 2004 |
pMCB17 | GFP gene (pyr4::alcA(p)::gfp2-5) in pUC19 | Fernandez-Abalos et al., 1998 |
pMCB17apx | pMCB17 version for fusion of GFP to N-termini of proteins of interest | V. P. Efimov, Piscataway, USA |
p17apx-apsB | 1.5 kb of apsB in PacI/AscI restriction site of pMCB17apx | V. P. Efimov, Piscataway, USA |
pRF280 | gpd(p)::stuA(NLS)::GFP; argB in pRS31 | R. Fischer, Marburg, Germany |
pRS31 | gpd(p)::stuA(NLS)::GFP in pBluescript KS— | Suelmann et al., 1997 |
pRS48 | apsB tagged with GFP in EcoRV in pBluescript KS— | Suelmann et al., 1997 |
pRS50 | alcA(p)::apsB::GFP pBluescript KS— | R. Suelman, Marburg, Germany |