Time taken from NEB to anaphase in control, Bub1 or BubR1-RNAi cultures exposed to no drugs, nocodazole or nocodazole plus ZM447439
| Drugs . | RNAi . | Median . | Mean . | s.e.m. . | n . | PA . |
|---|---|---|---|---|---|---|
| None | Control | 30 | 32 | 1 | 128 | 0 |
| Bub1 | 45 | 47 | 3 | 43 | 9 | |
| BubR1 | 20 | 20 | 1 | 48 | 27 | |
| Noc | Control | 558 | 497 | 68 | 6 | - |
| Bub1 | 388 | 377 | 34 | 32 | - | |
| BubR1 | 34 | 119 | 46 | 19 | - | |
| Noc + ZM | Control | 297 | 325 | 30 | 32 | - |
| Bub1 | 264 | 244 | 31 | 35 | - | |
| BubR1 | 84 | 213 | 70 | 11 | - | |
| Noc + ZM P1 | Bub1 | 41 | 42 | 5 | 12 | - |
| BubR1 | 34 | 53 | 12 | 7 | - | |
| P2 | Bub1 | 350 | 360 | 28 | 22 | - |
| BubR1 | 508 | 495 | 50 | 4 | - |
| Drugs . | RNAi . | Median . | Mean . | s.e.m. . | n . | PA . |
|---|---|---|---|---|---|---|
| None | Control | 30 | 32 | 1 | 128 | 0 |
| Bub1 | 45 | 47 | 3 | 43 | 9 | |
| BubR1 | 20 | 20 | 1 | 48 | 27 | |
| Noc | Control | 558 | 497 | 68 | 6 | - |
| Bub1 | 388 | 377 | 34 | 32 | - | |
| BubR1 | 34 | 119 | 46 | 19 | - | |
| Noc + ZM | Control | 297 | 325 | 30 | 32 | - |
| Bub1 | 264 | 244 | 31 | 35 | - | |
| BubR1 | 84 | 213 | 70 | 11 | - | |
| Noc + ZM P1 | Bub1 | 41 | 42 | 5 | 12 | - |
| BubR1 | 34 | 53 | 12 | 7 | - | |
| P2 | Bub1 | 350 | 360 | 28 | 22 | - |
| BubR1 | 508 | 495 | 50 | 4 | - |
The median and mean values represent the average time in minutes; s.e.m. represents the standard error of the mean; (n) is the number of cells analyzed; and PA is the number of cells that enter anaphase with unaligned chromosomes. P1 and P2 are the subpopulations shown in Fig. 3.