List of primers used in this work
| Primer sets used for RT-PCR | |||
| Gene product | Forward primer | Reverse primer | Product size (bp) |
| NcadΔC | AAACAGCCCTTCCTGCTACC | TCATGCTCAGAAGAGGAGACC | 195 |
| GAPDH | ACTTTGTGAAGCTCATTTCC | TGCAGCGAACTTTATTGATG | 265 |
| Primer sets used in real-time RT-PCR | |||
| Gene product | Forward primer | Reverse primer | |
| PPARγ2 | ACCACTCGCATTCCTTTGAC | TGGGTCAGCTCTTGTGAATG | |
| Adipsin | TGCATCAACTCAGAGGTGTCAATCA | TGCGCAGATTGCAGGTTGT | |
| Cbfa1 | CCGTGGCCTTCAAGGTTGT | TTCATAACAGCGGAGGCATTT | |
| Colα1(I) | GATGACGTGCAATGCAATGAA | TGATAGTATTCTTCCGGGCAGAAA | |
| Osteopontin | GTATTGCTTTTGCCTGTTTGG | TGAGCTGCCAGAATCAGTCAC | |
| Osteoprotegerin | TCCCGAGGACCACAATGAAC | TGGGTTGTCCATTCAATGATGT | |
| RANK-L | CCTGAGGCCCAGCCATTT | GGTACCAAGAGGACAGAGTGACTTTA |
| Primer sets used for RT-PCR | |||
| Gene product | Forward primer | Reverse primer | Product size (bp) |
| NcadΔC | AAACAGCCCTTCCTGCTACC | TCATGCTCAGAAGAGGAGACC | 195 |
| GAPDH | ACTTTGTGAAGCTCATTTCC | TGCAGCGAACTTTATTGATG | 265 |
| Primer sets used in real-time RT-PCR | |||
| Gene product | Forward primer | Reverse primer | |
| PPARγ2 | ACCACTCGCATTCCTTTGAC | TGGGTCAGCTCTTGTGAATG | |
| Adipsin | TGCATCAACTCAGAGGTGTCAATCA | TGCGCAGATTGCAGGTTGT | |
| Cbfa1 | CCGTGGCCTTCAAGGTTGT | TTCATAACAGCGGAGGCATTT | |
| Colα1(I) | GATGACGTGCAATGCAATGAA | TGATAGTATTCTTCCGGGCAGAAA | |
| Osteopontin | GTATTGCTTTTGCCTGTTTGG | TGAGCTGCCAGAATCAGTCAC | |
| Osteoprotegerin | TCCCGAGGACCACAATGAAC | TGGGTTGTCCATTCAATGATGT | |
| RANK-L | CCTGAGGCCCAGCCATTT | GGTACCAAGAGGACAGAGTGACTTTA |