Genetic partners of RacGAP(84C)
| Gene/predicted gene . | EP line . | cytology . | GG17 . | Molecular and functional information . | Rac1N17 . | Cdc42N17 . | RasN17 . | rn20 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A. Ras, Rac and Rho signalling proteins | ||||||||||||||||
| Misexpression of Ras 4 (MESR4) | EP(2)386 | 54C3-7 | Su | Suppressor of constitutive Ras-MAPK signalling | No effect | No effect | No effect | NT | ||||||||
| A kinase anchor protein (Akap 200) (syn.MESR2) | EP(2)2072 | 29C3-4 | Enh | Suppressor of constitutive Ras-MAPK signalling | No effect | No effect | No effect | NT | ||||||||
| EP(2)2254 | Enh | NT | NT | NT | NT | |||||||||||
| Drosophila C3G (DC3-G) | EP(1)1613* | 6D1-2 | Enh | RasGEF, activation of Ras-MAPK | NT | NT | NT | No effect | ||||||||
| UAS-DC3G | Enh | Enh | Enh | No effect | NT | |||||||||||
| CG31012 | EP(3)3700 | 100A6 | Enh | SH3 domain | No effect | No effect | No effect | NT | ||||||||
| pebble (pbl) | EP(3)3415* | 66A18 | Lethal | RhoGEF, functions in cytokinesis | Lethal | Enh (escapers) | No effect | Lethal | ||||||||
| GEF(64C) | EP(3)3035 | 64B14 | Enh | RhoGEF, functions in axonal growth | No effect | No effect | No effect | NT | ||||||||
| EP(3)3322* | Lethal | Lethal | Lethal | No effect | No effect | |||||||||||
| grapes (grp) | EP(2)587 | 36A10 | Enh | Ser/thr kinase, functions in DNA repair | Enh (rough) | Enh (rough) | Enh (glossy) | NT | ||||||||
| CG7097 | EP(2)2445 | 56C1-4 | Enh | Receptor signalling Ser/thr kinase | No effect | No effect | No effect | NT | ||||||||
| SNF/AMP-activated protein S/T kinase gamma subunit | EP(3)648 | 93C2-6 | Enh | AMP-activated Ser/thr kinase, functions as a metabolic sensor in yeast | Enh (escapers) | No effect | No effect | NT | ||||||||
| B. Cytoskeletal control and membrane trafficking | ||||||||||||||||
| Myo31DF | EP(2)2491 | 31F3 | Enh | Myosin ATPase | Enh | No effect | No effect | NT | ||||||||
| Beached1 (Beach1) | EP(2)2299* | 26A1 | Enh | Endocytic pathway, neuronal growth | Enh (escapers) | Enh (escapers) | Enh | Enh | ||||||||
| EP may drive a Nter truncated protein (see Table S1) | ||||||||||||||||
| C. DNA remodelling factors | ||||||||||||||||
| kismet (kis) | EP(2)474 | 21B2-4 | Enh | DNA helicase | No effect | No effect | NT | |||||||||
| EP(2)563 | Enh | No effect | No effect | No effect | ||||||||||||
| domino (dom) | EP(2)2371 | 57D11 | Enh | DNA helicase | No effect | No effect | No effect | |||||||||
| Dodeca-satellite-binding protein 1 (Dp1) | EP(2)2422 | 55D4 | Enh | Single strand DNA binding, KH domain | No effect | No effect | No effect | |||||||||
| D. Spermatogenesis | ||||||||||||||||
| purity of essence (poe) | EP(2)349 | 28E1 | Su | Male sterile, abnormal sperm tail | Su | Su | No effect | |||||||||
| EP(2)737 | Su | Su | Su | No effect | ||||||||||||
| Target of methylation-induced silencing 1 (TMS1d) | EP(3)807 | 72E5-F1 | Enh | Caspase-mediated cell death | No effect | No effect | No effect | |||||||||
| Centre divider (Cdi) | EP(3)3319 | 91E4-F1 | Su | Ser/thr kinase TESK1 | Su | No effect | No effect | |||||||||
| E. Others | ||||||||||||||||
| Traf2 | EP(1)325 | 7D16 | Enh | TNF-signalling pathway | No effect | No effect | No effect | |||||||||
| wunen2 | EP(2)652 | 45D | Enh | Phosphatidic acid phosphatase | No effect | No effect | No effect | |||||||||
| CG14959 | EP(2)714 | 63C1 | Enh | Chitin binding domain | NT | NT | NT | |||||||||
| EP(2)3139 | ||||||||||||||||
| CG5261 | EP(2)816 | 27F1-6 | Enh | Dihydrolipoamide-S-acetyl transferase | Enh | Enh | No effect | |||||||||
| CG3624 | EP(2)827 | 58D7 | Enh | Immunoglobulin domain | ||||||||||||
| rhomboid-6 (rho-6) | EP(2)2023 | 33D1 | Enh | EGF- MAP kinase signalling | No effect | No effect | No effect | |||||||||
| CG6701 | EP(2)2054 | 50C22 | lethal | C2H2 Zn finger domain | Enh | No effect | No effect | |||||||||
| CG8740 | EP(2)2233 | 44E3-4 | Enh | No putative conserved domain | Enh | Enh | No effect | |||||||||
| numb | EP(2)2455 | 30B5 | Su | Phosphotyrosine interaction and PH domains | Su | Su | No effect | |||||||||
| couch potato (cpo) | EP(3)3395 | 90C10 | Enh | RNA binding motifs | Enh | No effect | No effect | |||||||||
| EP(3)3608 | Enh | |||||||||||||||
| CG2017 | EP(3)3503 | 83C5-6 | Su | GTP-binding elongation factor | No effect | No effect | No effect | |||||||||
| Gene/predicted gene . | EP line . | cytology . | GG17 . | Molecular and functional information . | Rac1N17 . | Cdc42N17 . | RasN17 . | rn20 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A. Ras, Rac and Rho signalling proteins | ||||||||||||||||
| Misexpression of Ras 4 (MESR4) | EP(2)386 | 54C3-7 | Su | Suppressor of constitutive Ras-MAPK signalling | No effect | No effect | No effect | NT | ||||||||
| A kinase anchor protein (Akap 200) (syn.MESR2) | EP(2)2072 | 29C3-4 | Enh | Suppressor of constitutive Ras-MAPK signalling | No effect | No effect | No effect | NT | ||||||||
| EP(2)2254 | Enh | NT | NT | NT | NT | |||||||||||
| Drosophila C3G (DC3-G) | EP(1)1613* | 6D1-2 | Enh | RasGEF, activation of Ras-MAPK | NT | NT | NT | No effect | ||||||||
| UAS-DC3G | Enh | Enh | Enh | No effect | NT | |||||||||||
| CG31012 | EP(3)3700 | 100A6 | Enh | SH3 domain | No effect | No effect | No effect | NT | ||||||||
| pebble (pbl) | EP(3)3415* | 66A18 | Lethal | RhoGEF, functions in cytokinesis | Lethal | Enh (escapers) | No effect | Lethal | ||||||||
| GEF(64C) | EP(3)3035 | 64B14 | Enh | RhoGEF, functions in axonal growth | No effect | No effect | No effect | NT | ||||||||
| EP(3)3322* | Lethal | Lethal | Lethal | No effect | No effect | |||||||||||
| grapes (grp) | EP(2)587 | 36A10 | Enh | Ser/thr kinase, functions in DNA repair | Enh (rough) | Enh (rough) | Enh (glossy) | NT | ||||||||
| CG7097 | EP(2)2445 | 56C1-4 | Enh | Receptor signalling Ser/thr kinase | No effect | No effect | No effect | NT | ||||||||
| SNF/AMP-activated protein S/T kinase gamma subunit | EP(3)648 | 93C2-6 | Enh | AMP-activated Ser/thr kinase, functions as a metabolic sensor in yeast | Enh (escapers) | No effect | No effect | NT | ||||||||
| B. Cytoskeletal control and membrane trafficking | ||||||||||||||||
| Myo31DF | EP(2)2491 | 31F3 | Enh | Myosin ATPase | Enh | No effect | No effect | NT | ||||||||
| Beached1 (Beach1) | EP(2)2299* | 26A1 | Enh | Endocytic pathway, neuronal growth | Enh (escapers) | Enh (escapers) | Enh | Enh | ||||||||
| EP may drive a Nter truncated protein (see Table S1) | ||||||||||||||||
| C. DNA remodelling factors | ||||||||||||||||
| kismet (kis) | EP(2)474 | 21B2-4 | Enh | DNA helicase | No effect | No effect | NT | |||||||||
| EP(2)563 | Enh | No effect | No effect | No effect | ||||||||||||
| domino (dom) | EP(2)2371 | 57D11 | Enh | DNA helicase | No effect | No effect | No effect | |||||||||
| Dodeca-satellite-binding protein 1 (Dp1) | EP(2)2422 | 55D4 | Enh | Single strand DNA binding, KH domain | No effect | No effect | No effect | |||||||||
| D. Spermatogenesis | ||||||||||||||||
| purity of essence (poe) | EP(2)349 | 28E1 | Su | Male sterile, abnormal sperm tail | Su | Su | No effect | |||||||||
| EP(2)737 | Su | Su | Su | No effect | ||||||||||||
| Target of methylation-induced silencing 1 (TMS1d) | EP(3)807 | 72E5-F1 | Enh | Caspase-mediated cell death | No effect | No effect | No effect | |||||||||
| Centre divider (Cdi) | EP(3)3319 | 91E4-F1 | Su | Ser/thr kinase TESK1 | Su | No effect | No effect | |||||||||
| E. Others | ||||||||||||||||
| Traf2 | EP(1)325 | 7D16 | Enh | TNF-signalling pathway | No effect | No effect | No effect | |||||||||
| wunen2 | EP(2)652 | 45D | Enh | Phosphatidic acid phosphatase | No effect | No effect | No effect | |||||||||
| CG14959 | EP(2)714 | 63C1 | Enh | Chitin binding domain | NT | NT | NT | |||||||||
| EP(2)3139 | ||||||||||||||||
| CG5261 | EP(2)816 | 27F1-6 | Enh | Dihydrolipoamide-S-acetyl transferase | Enh | Enh | No effect | |||||||||
| CG3624 | EP(2)827 | 58D7 | Enh | Immunoglobulin domain | ||||||||||||
| rhomboid-6 (rho-6) | EP(2)2023 | 33D1 | Enh | EGF- MAP kinase signalling | No effect | No effect | No effect | |||||||||
| CG6701 | EP(2)2054 | 50C22 | lethal | C2H2 Zn finger domain | Enh | No effect | No effect | |||||||||
| CG8740 | EP(2)2233 | 44E3-4 | Enh | No putative conserved domain | Enh | Enh | No effect | |||||||||
| numb | EP(2)2455 | 30B5 | Su | Phosphotyrosine interaction and PH domains | Su | Su | No effect | |||||||||
| couch potato (cpo) | EP(3)3395 | 90C10 | Enh | RNA binding motifs | Enh | No effect | No effect | |||||||||
| EP(3)3608 | Enh | |||||||||||||||
| CG2017 | EP(3)3503 | 83C5-6 | Su | GTP-binding elongation factor | No effect | No effect | No effect | |||||||||
Eye directed expression of EP-associated genes with: the GAP domain of RacGAP(84C) (GG17) (column 4), Rac1N17 (column 6), Cdc42N17 (column 7), RasN17 (column 8); or in rn20 hemizygous context (last column, A,B).
NT, not tested.
EP lines presenting a rough eye in combination with GMRGal4.