Oligonucleotide primer sequences
| Gene . | Accession number . | Forward primer . | Reverse primer . | Product (bp) . |
|---|---|---|---|---|
| Dyn1 | X54531 | 5′-GAC CAG ATC GAC ACT TAT GAA-3′ | 5′-GCC ACC CCT TTC GAA TG-3′ | 533 |
| Dyn2 | L24562 | 5′-ATC AAG TCG ACA CCC TGG AG-3′ | 5′-ACC AGC CCC TGC GGA TC-3′ | 531 |
| Dyn3 | D14076 | 5′-CCA CCA CCC AAA GAA GGC T-3′ | 5′-CTG GAT GGA ACC TGT GGA G-3′ | 190 |
| Amph1 | Y13381 | 5′-CTG GAG GTG AAG AAG GAG G-3′ | 5′-TCA GTT CCC ACA GTC TCA CC-3′ | 398 |
| Amph2 | Y13380 | 5′-GGA TGG CAG AGA TGG GGA GCA AGG G-3′ | 5′-ATG GAA GTT TTC CTC CAG ACC CGC GAT GC-3′ | * |
| Amph2 full | Y13380 | 5′-GGA TGG CAG AGA TGG GGA GCA AGG G-3′ | 5′-TCA CAC ACC GGA AGG CTG CAG AGG C-3′ | * |
| Gene . | Accession number . | Forward primer . | Reverse primer . | Product (bp) . |
|---|---|---|---|---|
| Dyn1 | X54531 | 5′-GAC CAG ATC GAC ACT TAT GAA-3′ | 5′-GCC ACC CCT TTC GAA TG-3′ | 533 |
| Dyn2 | L24562 | 5′-ATC AAG TCG ACA CCC TGG AG-3′ | 5′-ACC AGC CCC TGC GGA TC-3′ | 531 |
| Dyn3 | D14076 | 5′-CCA CCA CCC AAA GAA GGC T-3′ | 5′-CTG GAT GGA ACC TGT GGA G-3′ | 190 |
| Amph1 | Y13381 | 5′-CTG GAG GTG AAG AAG GAG G-3′ | 5′-TCA GTT CCC ACA GTC TCA CC-3′ | 398 |
| Amph2 | Y13380 | 5′-GGA TGG CAG AGA TGG GGA GCA AGG G-3′ | 5′-ATG GAA GTT TTC CTC CAG ACC CGC GAT GC-3′ | * |
| Amph2 full | Y13380 | 5′-GGA TGG CAG AGA TGG GGA GCA AGG G-3′ | 5′-TCA CAC ACC GGA AGG CTG CAG AGG C-3′ | * |
These primer pairs produced several bands due to alternative splicing.