The Company of Biologists

Table 2.

List of genomic clones containing SLMAP exons and the methods of isolation

Clone (size)	Exons encompassed	Method obtained
A (10 kb)	Exon II	Direct screening of genomic library^*
B (3.6 kb)	Exon IV to Exon V	PCR cloning (708-F & 822-R)^{^†}
C (2.0 kb)	Exon V to Exon VI	PCR cloning (781-F & 851-R)^{^†}
D (3.0 kb)	Exon VI to Exon II	PCR cloning (829-F & 966-R)^{^†}
E (6.0 kb)	Exon VII to Exon IX	PCR cloning (922-F & 1377-R)^{^†}
F (4.0 kb)	Exon XIV to Exon XVI	PCR cloning (Ex1-F & Ex3-R)^{^†}
G (20 kb)	Exon XV to Exon XXI	Direct screening of genomic library^{^‡}
H (4.5 kb)	Exon XIII to Exon XV	Direct screening of genomic library^{^‡}

Clone (size)	Exons encompassed	Method obtained
A (10 kb)	Exon II	Direct screening of genomic library^*
B (3.6 kb)	Exon IV to Exon V	PCR cloning (708-F & 822-R)^{^†}
C (2.0 kb)	Exon V to Exon VI	PCR cloning (781-F & 851-R)^{^†}
D (3.0 kb)	Exon VI to Exon II	PCR cloning (829-F & 966-R)^{^†}
E (6.0 kb)	Exon VII to Exon IX	PCR cloning (922-F & 1377-R)^{^†}
F (4.0 kb)	Exon XIV to Exon XVI	PCR cloning (Ex1-F & Ex3-R)^{^†}
G (20 kb)	Exon XV to Exon XXI	Direct screening of genomic library^{^‡}
H (4.5 kb)	Exon XIII to Exon XV	Direct screening of genomic library^{^‡}

λ FIX II mouse genomic library (Stratagene).

The exon-intron junctions of SLMAP3 and sizes of the respective exons and introns.

λ DASH II mouse genomic library (Stratagene); see Table 3.