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Table 4.

Comparison of features distinguishing plateins from other articulins

ArticulinsPlateins
Cellular location
 
Cytoplasmic (sub-plasmalemmal)
 
Within membranous cisternae (intra-alveolar)
 
N-terminal signal sequence
 
Absent
 
Present
 
Major repetitive domain (`core')
 
VP-rich 12-mers (VPVPV...)
 
VP-rich 12-mers (VP-charged)
 
Second repetitive domain (number of repeats) Pseudomicrothorax dubius5-mers (P-rich, no G) 
 
  • 6-mers (G-rich), C-terminal (9-13)

 
 
 Euglena gracilisα1,2: N-terminal (15; 14) 

 
  • Artic 80: 7-mers (APVT...), C-terminal (4)

  • Artic 86: 7-mers (APVT...), N-terminal (4)


 
β/γ: C-terminal (17)
 
Charged amino acids: total (%) (net charge) P. dubiusα1: 24.3% (-47) 
 
  • Artic 1: 13.9% (+1)

  • Artic 4: 16.1% (-12)

 
α2: 24.0% (-39) 
 E. gracilisβ/γ: 29.4% (-34) 

 
  • Artic 80: 19.2% (+1)

  • Artic 86: 21.2% (+2)


 

 
Predicted pI: entire protein (`core' domain only) P. dubiusα1: 4.75 (4.43) 
 
  • Artic 1: 7.91 (5.02)

  • Artic 4: 5.30 (4.85)

 
α2: 4.77 (4.39) 
 E. gracilisβ/γ: 4.88 (4.66) 

 
  • Artic 80: 7.86 (7.07)

  • Artic 86: 8.19 (8.71)


 

 
Anomalous gel retardation (gene-derived versus SDS-PAGE Mrs; `n'=apparent gel MrP. dubius 
 
  • 69.7 →→ `78-80' kDa

  • 59.9 →→ `78-80'

 
α: 56.3-60.9 →→ `125' kDa 
 E. gracilisβ/γ: 72.8 →→ `95-99' 
 
  • 72.1 →→ `80'

  • 71.9 →→ `86'

 
 
ArticulinsPlateins
Cellular location
 
Cytoplasmic (sub-plasmalemmal)
 
Within membranous cisternae (intra-alveolar)
 
N-terminal signal sequence
 
Absent
 
Present
 
Major repetitive domain (`core')
 
VP-rich 12-mers (VPVPV...)
 
VP-rich 12-mers (VP-charged)
 
Second repetitive domain (number of repeats) Pseudomicrothorax dubius5-mers (P-rich, no G) 
 
  • 6-mers (G-rich), C-terminal (9-13)

 
 
 Euglena gracilisα1,2: N-terminal (15; 14) 

 
  • Artic 80: 7-mers (APVT...), C-terminal (4)

  • Artic 86: 7-mers (APVT...), N-terminal (4)


 
β/γ: C-terminal (17)
 
Charged amino acids: total (%) (net charge) P. dubiusα1: 24.3% (-47) 
 
  • Artic 1: 13.9% (+1)

  • Artic 4: 16.1% (-12)

 
α2: 24.0% (-39) 
 E. gracilisβ/γ: 29.4% (-34) 

 
  • Artic 80: 19.2% (+1)

  • Artic 86: 21.2% (+2)


 

 
Predicted pI: entire protein (`core' domain only) P. dubiusα1: 4.75 (4.43) 
 
  • Artic 1: 7.91 (5.02)

  • Artic 4: 5.30 (4.85)

 
α2: 4.77 (4.39) 
 E. gracilisβ/γ: 4.88 (4.66) 

 
  • Artic 80: 7.86 (7.07)

  • Artic 86: 8.19 (8.71)


 

 
Anomalous gel retardation (gene-derived versus SDS-PAGE Mrs; `n'=apparent gel MrP. dubius 
 
  • 69.7 →→ `78-80' kDa

  • 59.9 →→ `78-80'

 
α: 56.3-60.9 →→ `125' kDa 
 E. gracilisβ/γ: 72.8 →→ `95-99' 
 
  • 72.1 →→ `80'

  • 71.9 →→ `86'

 
 

Articulins described from the ciliate P. dubius(Huttenlauch et al., 1998a)and the euglenoid flagellate E. gracilis(Marrs and Bouck, 1992) are used to represent the standard articulins. Amino acid composition and pI predictions for entire proteins (minus signal sequences for the plateins) and the major repeat core domains were derived using the ProtParam tool (ExPASy proteomics server of the Swiss Institute of Bioinformatics).

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