Analysis of amino acid use within the primary 12-mer repetitive domains of plateins, with articulin 1 of Pseudomicrothorax dubius for comparison
Articulin 1 (P. dubius) (30 central repeats) | ||||||||||||||||||||||||||
Consensus | V | P | V | P | V | P | V | P | V | P | V | P | 360 | |||||||||||||
+ | 4 | 0 | 2 | 0 | 7 | 1 | 6 | 0 | 3 | 1 | 4 | 3 | +31 | |||||||||||||
- | 0 | 10 | 1 | 9 | 0 | 5 | 0 | 7 | 1 | 5 | 0 | 4 | -42 | |||||||||||||
Charge consensus | + | - | (+) | - | + | - | + | - | + | - | + | (-) | 73 (-11) | |||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||||||||||
α-Platein-1 (E. aediculatus) (28 C-terminal repeats) | ||||||||||||||||||||||||||
Consensus | V | P | R/H | T | Y | E | Y | Q/V | E | Z | I/V | - | 336 | |||||||||||||
+ | 0 | 0 | 13 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 0 | +18 | |||||||||||||
- | 0 | 0 | 0 | 5 | 1 | 17 | 3 | 5 | 20 | 13 | 0 | 2 | -66 | |||||||||||||
Charge consensus | + | - | (-) | - | - | - | - | (+) | (-) | 84 (-48) | ||||||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||||||||||
α-Platein-2 (E. aediculatus) (24 C-terminal repeats) | ||||||||||||||||||||||||||
Consensus | V | P | R/H | T | Y/V | E | Y | Q/V | E | Z | V/I | - | 288 | |||||||||||||
+ | 0 | 0 | 13 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | +17 | |||||||||||||
- | 0 | 0 | 0 | 5 | 0 | 15 | 2 | 5 | 18 | 12 | 0 | 2 | -59 | |||||||||||||
Charge consensus | + | - | - | - | - | - | (+) | (-) | 76 (-42) | |||||||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||||||||||
β/γ-Platein (E. aediculatus) (27 central repeats) | ||||||||||||||||||||||||||
Consensus | V | P/D | E | V/P | E | Y | R/V | T | R | Y | Z | T/V | 324 | |||||||||||||
+ | 0 | 1 | 0 | 1 | 3 | 4 | 15 | 2 | 16 | 0 | 0 | 0 | +42 | |||||||||||||
- | 0 | 9 | 27 | 1 | 14 | 3 | 0 | 1 | 1 | 1 | 12 | 1 | -70 | |||||||||||||
Charge consensus | - | - | - | (+) | + | (+) | + | (-) | - | (-) | 112 (-28) | |||||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Articulin 1 (P. dubius) (30 central repeats) | ||||||||||||||||||||||||||
Consensus | V | P | V | P | V | P | V | P | V | P | V | P | 360 | |||||||||||||
+ | 4 | 0 | 2 | 0 | 7 | 1 | 6 | 0 | 3 | 1 | 4 | 3 | +31 | |||||||||||||
- | 0 | 10 | 1 | 9 | 0 | 5 | 0 | 7 | 1 | 5 | 0 | 4 | -42 | |||||||||||||
Charge consensus | + | - | (+) | - | + | - | + | - | + | - | + | (-) | 73 (-11) | |||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||||||||||
α-Platein-1 (E. aediculatus) (28 C-terminal repeats) | ||||||||||||||||||||||||||
Consensus | V | P | R/H | T | Y | E | Y | Q/V | E | Z | I/V | - | 336 | |||||||||||||
+ | 0 | 0 | 13 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 0 | +18 | |||||||||||||
- | 0 | 0 | 0 | 5 | 1 | 17 | 3 | 5 | 20 | 13 | 0 | 2 | -66 | |||||||||||||
Charge consensus | + | - | (-) | - | - | - | - | (+) | (-) | 84 (-48) | ||||||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||||||||||
α-Platein-2 (E. aediculatus) (24 C-terminal repeats) | ||||||||||||||||||||||||||
Consensus | V | P | R/H | T | Y/V | E | Y | Q/V | E | Z | V/I | - | 288 | |||||||||||||
+ | 0 | 0 | 13 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | +17 | |||||||||||||
- | 0 | 0 | 0 | 5 | 0 | 15 | 2 | 5 | 18 | 12 | 0 | 2 | -59 | |||||||||||||
Charge consensus | + | - | - | - | - | - | (+) | (-) | 76 (-42) | |||||||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||||||||||
β/γ-Platein (E. aediculatus) (27 central repeats) | ||||||||||||||||||||||||||
Consensus | V | P/D | E | V/P | E | Y | R/V | T | R | Y | Z | T/V | 324 | |||||||||||||
+ | 0 | 1 | 0 | 1 | 3 | 4 | 15 | 2 | 16 | 0 | 0 | 0 | +42 | |||||||||||||
- | 0 | 9 | 27 | 1 | 14 | 3 | 0 | 1 | 1 | 1 | 12 | 1 | -70 | |||||||||||||
Charge consensus | - | - | - | (+) | + | (+) | + | (-) | - | (-) | 112 (-28) | |||||||||||||||
Residue No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Consensus residues at each position within the 12-mer repeat (bold) are those whose frequency at that position is at least twice that of the next most common residue (minimum of 10 uses for the consensus residue). Charged residues (+=R or K; -=D or E) at each position are also noted, and summed for all repeats in the right-hand column. Notable are the deviation of the plateins from the VPVPV... consensus and the strict alternation of positively and negatively charged residues (substituting for V and P, respectively, at particular repeat positions) of articulin. Note also the very large excess of negatively charged residues (red numerals) in plateins, compared with articulin. (Z=E or Q).