Nucleotide sequences of the primers used for PCR amplifications of the Euplotes aediculatus α-platein genes
| Primer name . | Nucleotide sequence (5′-3′) . | Origin/specificity (peptide) . |
|---|---|---|
| AP-1 | TAT GTI GCI CCI GCI GGI CCI CCI TAT GG | f30 (a.a. 6-14) |
| AP-2* | CTA TAI CCI CCI GCI GGI GCI ACA TA | f30 (a.a. 6-14) |
| AP-3 | GTI TTT ACI TAT ACI GAT GAI CAT CAT AC | f55 (a.a. 5-14) |
| AP-4* | GTA TGA TGI TCA TCI GTA TAI GTA AAI AC | f55 (a.a. 5-14) |
| AP-5 | CCI TAT ACI TAT GAI TAT GTI GAT GT | f55 (a.a 15-24) |
| AP-6* | ACA TCI ACI ACA TAI TCA TAI GTA TAI GG | f55 (a.a 15-24) |
| AP-7 | ATG GTA CCA CAT CAA TGA CAA ATT | α1 (n.t. 1236-1259) |
| AP-8* | AGG AAC GTG GAT TTC TTC GAC | α1 (n.t. 888-908) |
| AP-9 | GAC CGC TCA GCC AGT AAC AAC CG | α2 (n.t. 152-174) |
| AP-10* | TTA CCA TCT TCT GTA TCC ACC | α1 (n.t. 1680-1700); and α2 (n.t. 1572-1592) |
| AP-11* | TTG GAA CGC TAA CTT CTT CAA CA | α1 (n.t. 1079-1101; and α2 (n.t. 971-993) |
| AP-12 | AAG GCC GCA CTA CCA CCA GGT C | α1 (n.t. 611-632) |
| AP-13 | CAC CAA AGC AAA AGT ACC ACT ATT CA | α2 (n.t. 619-644) |
| AP-14 | TGT TGA AGA AGT TAG CGT TCC AA | α1 (n.t. 1079-1101); and α2 (n.t. 971-993) |
| 5′α1TEL | GTT ATA TTG ATA ATG GGT TTT AAG | α1 (n.t. 40-63) |
| 3′α1TEL* | CTC GAT AAA ATG TTT GCG CAT TTA | α1 (n.t. 1941-1964) |
| C4A4TEL | CCC CAA AAC CCC AAA ACC CC | (C4A4) 5′-telomeres |
| Primer name . | Nucleotide sequence (5′-3′) . | Origin/specificity (peptide) . |
|---|---|---|
| AP-1 | TAT GTI GCI CCI GCI GGI CCI CCI TAT GG | f30 (a.a. 6-14) |
| AP-2* | CTA TAI CCI CCI GCI GGI GCI ACA TA | f30 (a.a. 6-14) |
| AP-3 | GTI TTT ACI TAT ACI GAT GAI CAT CAT AC | f55 (a.a. 5-14) |
| AP-4* | GTA TGA TGI TCA TCI GTA TAI GTA AAI AC | f55 (a.a. 5-14) |
| AP-5 | CCI TAT ACI TAT GAI TAT GTI GAT GT | f55 (a.a 15-24) |
| AP-6* | ACA TCI ACI ACA TAI TCA TAI GTA TAI GG | f55 (a.a 15-24) |
| AP-7 | ATG GTA CCA CAT CAA TGA CAA ATT | α1 (n.t. 1236-1259) |
| AP-8* | AGG AAC GTG GAT TTC TTC GAC | α1 (n.t. 888-908) |
| AP-9 | GAC CGC TCA GCC AGT AAC AAC CG | α2 (n.t. 152-174) |
| AP-10* | TTA CCA TCT TCT GTA TCC ACC | α1 (n.t. 1680-1700); and α2 (n.t. 1572-1592) |
| AP-11* | TTG GAA CGC TAA CTT CTT CAA CA | α1 (n.t. 1079-1101; and α2 (n.t. 971-993) |
| AP-12 | AAG GCC GCA CTA CCA CCA GGT C | α1 (n.t. 611-632) |
| AP-13 | CAC CAA AGC AAA AGT ACC ACT ATT CA | α2 (n.t. 619-644) |
| AP-14 | TGT TGA AGA AGT TAG CGT TCC AA | α1 (n.t. 1079-1101); and α2 (n.t. 971-993) |
| 5′α1TEL | GTT ATA TTG ATA ATG GGT TTT AAG | α1 (n.t. 40-63) |
| 3′α1TEL* | CTC GAT AAA ATG TTT GCG CAT TTA | α1 (n.t. 1941-1964) |
| C4A4TEL | CCC CAA AAC CCC AAA ACC CC | (C4A4) 5′-telomeres |
Indicates reverse primers.