. | TPO . | SCF . | FL . | G-CSF . | CHL-S . | CHL-L . | IL-1β . | IL-3 . | IL-6 . | IL-11 . | LIF . | OSM . | TGF-β1 . | . |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 516† . | 586 . | 603 . | 515 . | 979 . | 750 . | 330 . | 400 . | 420 . | 485 . | 520 . | 350 . | 295 . | β-actin . |
FBMD-1 | 2 | 4 | 3 | 4 | 2 | 3 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 3 |
M2-10B4 | 2 | 3 | 3 | 3 | 0 | 2 | nd | nd | nd | nd | nd | nd | nd | 3 |
MS-5 | 3 | 4 | 3 | 4 | 0 | 2 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM14-1C4* | 2 | 3 | 2 | 3 | 3 | 3 | 2 | 0 | 2 | 2 | 0 | 0 | 3 | 3 |
AM20-6B4 | 0 | 1 | 1 | 0 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM20-3C3 | 0 | 3 | 2 | 2 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM30-1C6 | 3 | 2 | 1 | 1 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM30-2A4 | 0 | 3 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 3 |
AM30-3A3 | 2 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 3 |
AM30-3E8 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
AM30-3F4 | 2 | 3 | 2 | 2 | 2 | 2 | 1 | 0 | 1 | 2 | 0 | 0 | 3 | 3 |
AM30-3F5 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 |
UG07-1C6* | 3 | 1 | 1 | 1 | 0 | 0 | nd | nd | nd | nd | nd | nd | nd | 3 |
UG15-1B7* | 0 | 1 | 0 | 0 | 0 | 0 | nd | nd | nd | nd | nd | nd | nd | 1 |
UG26-1B4 | 0 | 2 | 1 | 1 | 2 | 2 | nd | 0 | nd | nd | 1 | 0 | 2 | 3 |
UG26-1B6 | 2 | 3 | 1 | 2 | 2 | 2 | 2 | 0 | 3 | 0 | 1 | 0 | 3 | 3 |
UG26-2B3 | 2 | 2 | 1 | 2 | 0 | 0 | nd | 0 | nd | nd | 2 | 0 | 3 | 3 |
UG26.3B5 | nd | nd | nd | nd | nd | nd | 0 | 0 | 2 | 1 | 2 | 0 | 3 | nd |
UG26-2D3 | 3 | 3 | 1 | 2 | 3 | 3 | 1 | 0 | 3 | 2 | 0 | 0 | 1 | 3 |
UG26-3D4 | 1 | 3 | 1 | 2 | 0 | 1 | 2 | 0 | 3 | 1 | 1 | 0 | 2 | 3 |
EL08-1D2* | 3 | 3 | 3 | 2 | 3 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 |
EL08-2C2* | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
EL23-1B4 | 2 | 3 | 2 | 2 | 1 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
EL28-1B3 | 2 | 2 | 1 | 2 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
GI09-2E6* | 2 | 2 | 1 | 2 | 3 | 3 | 1 | 0 | 1 | 1 | 2 | 0 | 3 | 3 |
GI29-2B4 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 3 | 3 |
. | TPO . | SCF . | FL . | G-CSF . | CHL-S . | CHL-L . | IL-1β . | IL-3 . | IL-6 . | IL-11 . | LIF . | OSM . | TGF-β1 . | . |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 516† . | 586 . | 603 . | 515 . | 979 . | 750 . | 330 . | 400 . | 420 . | 485 . | 520 . | 350 . | 295 . | β-actin . |
FBMD-1 | 2 | 4 | 3 | 4 | 2 | 3 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 3 |
M2-10B4 | 2 | 3 | 3 | 3 | 0 | 2 | nd | nd | nd | nd | nd | nd | nd | 3 |
MS-5 | 3 | 4 | 3 | 4 | 0 | 2 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM14-1C4* | 2 | 3 | 2 | 3 | 3 | 3 | 2 | 0 | 2 | 2 | 0 | 0 | 3 | 3 |
AM20-6B4 | 0 | 1 | 1 | 0 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM20-3C3 | 0 | 3 | 2 | 2 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM30-1C6 | 3 | 2 | 1 | 1 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
AM30-2A4 | 0 | 3 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 3 |
AM30-3A3 | 2 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 3 |
AM30-3E8 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
AM30-3F4 | 2 | 3 | 2 | 2 | 2 | 2 | 1 | 0 | 1 | 2 | 0 | 0 | 3 | 3 |
AM30-3F5 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 |
UG07-1C6* | 3 | 1 | 1 | 1 | 0 | 0 | nd | nd | nd | nd | nd | nd | nd | 3 |
UG15-1B7* | 0 | 1 | 0 | 0 | 0 | 0 | nd | nd | nd | nd | nd | nd | nd | 1 |
UG26-1B4 | 0 | 2 | 1 | 1 | 2 | 2 | nd | 0 | nd | nd | 1 | 0 | 2 | 3 |
UG26-1B6 | 2 | 3 | 1 | 2 | 2 | 2 | 2 | 0 | 3 | 0 | 1 | 0 | 3 | 3 |
UG26-2B3 | 2 | 2 | 1 | 2 | 0 | 0 | nd | 0 | nd | nd | 2 | 0 | 3 | 3 |
UG26.3B5 | nd | nd | nd | nd | nd | nd | 0 | 0 | 2 | 1 | 2 | 0 | 3 | nd |
UG26-2D3 | 3 | 3 | 1 | 2 | 3 | 3 | 1 | 0 | 3 | 2 | 0 | 0 | 1 | 3 |
UG26-3D4 | 1 | 3 | 1 | 2 | 0 | 1 | 2 | 0 | 3 | 1 | 1 | 0 | 2 | 3 |
EL08-1D2* | 3 | 3 | 3 | 2 | 3 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 |
EL08-2C2* | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
EL23-1B4 | 2 | 3 | 2 | 2 | 1 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
EL28-1B3 | 2 | 2 | 1 | 2 | 0 | 1 | nd | nd | nd | nd | nd | nd | nd | 3 |
GI09-2E6* | 2 | 2 | 1 | 2 | 3 | 3 | 1 | 0 | 1 | 1 | 2 | 0 | 3 | 3 |
GI29-2B4 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 3 | 3 |
Stromal clones derived from BL1b transgenic embryos. All other stromal clones are derived from tsA58 transgenic embryos. †Fragment size of RT-PCR product. Signal intensity: 4,extremely high; 3, high; 2, intermediate; 1, low; 0, no expression; nd, not determined. RNA was prepared from non-irradiated stromal cells, and normalization with β-actin signal was performed for TPO, SCF, FL, G-CSF,CHL-S and -L RT-PCRs. RNAs for the remaining RT-PCRs were from irradiated stromal clones, and the GAPDH signal (not shown) was used for normalization. Signal intensities were compared only within a PCR set and not between PCR sets.