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Table 1.

Yeast strains used in this study

Strain	Genotype	Source
CRY3	MAT α Sec⁺can1-100 ade 2-1 his2-11, 15 leu2-3-112 trp1-1 ura3-1/MATa can1-100 ade2-1 his2-11, 15 leu2-3-112 trp1-1 ura3-1	R.S. Fuller^{^*}
NY13	MATa Sec⁺ura3-52	P. Novick^{^†}
NY431	MATa ura3-52 sec18-1	P. Novick^{^†}
X2180-1A	MATa Sec⁺SUC2 mal gal2 CUP1	Yeast Genetic Stock Center
SF266-1C	MATa SUC2 mal gal2 CUP1 sec12-4	Yeast Genetic Stock Center
SEY6210	MAT α leu2-3, 112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9	S. Emr^{^‡}
SEY6210 Δvps4	MAT α leu2-3, 112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 vps4::TRP1	S. Emr^{^‡}
SEY6210 Δvps8	MAT α leu2-3, 112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 vps8::LEU2	S. Emr^{^‡}
RH1800	END4	H. Riezman^{^§}
RH1800 end4-1	end4-1	H. Riezman^{^§}
RH448	MAT α his4 leu2 ura3 lys2 bar1	H. Riezman^{^§}
RH1966/299-1D	MATa end4::LEU2 his4 leu2 ura3 lys2 bar1	H. Riezman^{^§}

Strain	Genotype	Source
CRY3	MAT α Sec⁺can1-100 ade 2-1 his2-11, 15 leu2-3-112 trp1-1 ura3-1/MATa can1-100 ade2-1 his2-11, 15 leu2-3-112 trp1-1 ura3-1	R.S. Fuller^{^*}
NY13	MATa Sec⁺ura3-52	P. Novick^{^†}
NY431	MATa ura3-52 sec18-1	P. Novick^{^†}
X2180-1A	MATa Sec⁺SUC2 mal gal2 CUP1	Yeast Genetic Stock Center
SF266-1C	MATa SUC2 mal gal2 CUP1 sec12-4	Yeast Genetic Stock Center
SEY6210	MAT α leu2-3, 112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9	S. Emr^{^‡}
SEY6210 Δvps4	MAT α leu2-3, 112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 vps4::TRP1	S. Emr^{^‡}
SEY6210 Δvps8	MAT α leu2-3, 112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 vps8::LEU2	S. Emr^{^‡}
RH1800	END4	H. Riezman^{^§}
RH1800 end4-1	end4-1	H. Riezman^{^§}
RH448	MAT α his4 leu2 ura3 lys2 bar1	H. Riezman^{^§}
RH1966/299-1D	MATa end4::LEU2 his4 leu2 ura3 lys2 bar1	H. Riezman^{^§}

^*

University of Michegan, Ann Arbor, MI.

Yale University, New Haven, CT.

University of California - San Diego, La Jolla, CA.

University of Basel, Basel, Switzerland.