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Table 1.

Depletion of cytoplasmic TRAF2-GFP, cIAP1-GFP and cIAP2-GFP after TNF-R2 stimulation

Experiment no.Depletion of TRAF2-GFP (%)Depletion of cIAP1-GFP (%)Depletion of cIAP2-GFP (%)
26 45 81 
36 69 57 
37 73 66 
37 47 55 
39 61 52 
45 70 27 
Average 37 52 56 
Experiment no.Depletion of TRAF2-GFP (%)Depletion of cIAP1-GFP (%)Depletion of cIAP2-GFP (%)
26 45 81 
36 69 57 
37 73 66 
37 47 55 
39 61 52 
45 70 27 
Average 37 52 56 

To calculate the depletion of cytoplasmic fluorescent proteins,fluorescence intensities in a cytoplasmic area were determined before and 30 minutes after receptor stimulation (αTNF-R2). All measurements were corrected for bleaching effects, which were obtained by analyses of nonstimulated cells. Bleaching effects in all experiments were between 3 and 12% with an average of 7.3%. Depletion of TRAF2-GFP was determined after transfection of a 1:1 mixture of a TRAF2-GFP expression plasmid and empty vector, and depletion of cIAP1/2-GFP was determined after transfection with a 1:1 mixture of expression plasmids encoding TRAF2 and cIAP1/2-GFP.

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