Recovery of fetal liver cells following cryopreservation
| Fetal liver number . | State of cells before cryopreservation . | Cells viable after recovery (>80% attached on plastic culture dishes) . | Long-term cultures established after cells thawed (>three passages) . |
|---|---|---|---|
| #1210972 | Primary cells | Yes | Yes |
| #21198 | Primary cells | Yes | Yes |
| #32598 | Primary cells | Yes | Yes |
| #32598 | 2nd passage (P2) | Yes | Yes |
| #32598 | 4th passage (P4) | Yes | Yes |
| #4298 | Primary cells | Yes | Yes |
| #43098 | Primary cells | Yes | Yes |
| #43098 | 1st passage (P1) | Yes | Yes |
| #43098 | 3rd passage (P3) | Yes | Yes |
| #43098 | 8th passage (P8) | Yes | Yes |
| #210991 | Primary cells | Yes | Yes |
| #210991 | 2nd passage (P2) | Yes | Yes |
| #210991 | 6th passage (P6) | Yes | Yes |
| #9899 | Primary cells | Yes | Yes |
| #9899 | 3rd passage (P3) | Yes | Yes |
| #9899 | 4th passage (P4) | Yes | Yes |
| #9899 | 6th passage (P6) | Yes | Yes |
| Fetal liver number . | State of cells before cryopreservation . | Cells viable after recovery (>80% attached on plastic culture dishes) . | Long-term cultures established after cells thawed (>three passages) . |
|---|---|---|---|
| #1210972 | Primary cells | Yes | Yes |
| #21198 | Primary cells | Yes | Yes |
| #32598 | Primary cells | Yes | Yes |
| #32598 | 2nd passage (P2) | Yes | Yes |
| #32598 | 4th passage (P4) | Yes | Yes |
| #4298 | Primary cells | Yes | Yes |
| #43098 | Primary cells | Yes | Yes |
| #43098 | 1st passage (P1) | Yes | Yes |
| #43098 | 3rd passage (P3) | Yes | Yes |
| #43098 | 8th passage (P8) | Yes | Yes |
| #210991 | Primary cells | Yes | Yes |
| #210991 | 2nd passage (P2) | Yes | Yes |
| #210991 | 6th passage (P6) | Yes | Yes |
| #9899 | Primary cells | Yes | Yes |
| #9899 | 3rd passage (P3) | Yes | Yes |
| #9899 | 4th passage (P4) | Yes | Yes |
| #9899 | 6th passage (P6) | Yes | Yes |