Degenerate (upper case) and specific (lower case) primers used in cloning T. marmoratus opsins
| Primers | Sequence 5′>3′ | Size |
| LWFw I | GAACCTGGCCTTCTCCGAYTTYNKNAT | 27 |
| LWRv I | ACGTTCATCTTCTTGGCCTGYTCNYKCAT | 29 |
| LWFw II | TTCGACCGGTACAACGTGATHGTNMANGG | 29 |
| LWRv II | CAGGTAGGGGGTCCAGGCVAWRAACCA | 27 |
| BIFw | CATGTTCATCATCAACCTGGCNATHTTYGA | 30 |
| BIRv | CGAAGGAGCAGGTGGACARRWANCCYTC | 28 |
| UVFw I | GAGCTGATGCACATCCCCSARCAYTGGYT | 29 |
| UVRv I | CGAAGGTGTCGGTCAGGTAGTCRAANSWRCA | 31 |
| UVFw II | TGGTCAAGACCCCCATCTTYAYGYAYAA | 28 |
| UVRv II | CAGATGGTGATGGCGGCYTTNGCDATNC | 28 |
| 3′ RACE LW | tgatgaccatctcgctgtggttct | 24 |
| 5′ RACE LW | aggtctcgtagtagcagttgacg | 23 |
| 3′ RACE UV I | cgaagcaaccagaatcaacagacc | 24 |
| 5′ RACE UV I | tagctccgatacctgataaagagc | 24 |
| 3′ RACE UV II | tattctgcctgctatggaagtct | 23 |
| 5′ RACE UV II | acctcggttgaatgagttgtaga | 23 |
| Primers | Sequence 5′>3′ | Size |
| LWFw I | GAACCTGGCCTTCTCCGAYTTYNKNAT | 27 |
| LWRv I | ACGTTCATCTTCTTGGCCTGYTCNYKCAT | 29 |
| LWFw II | TTCGACCGGTACAACGTGATHGTNMANGG | 29 |
| LWRv II | CAGGTAGGGGGTCCAGGCVAWRAACCA | 27 |
| BIFw | CATGTTCATCATCAACCTGGCNATHTTYGA | 30 |
| BIRv | CGAAGGAGCAGGTGGACARRWANCCYTC | 28 |
| UVFw I | GAGCTGATGCACATCCCCSARCAYTGGYT | 29 |
| UVRv I | CGAAGGTGTCGGTCAGGTAGTCRAANSWRCA | 31 |
| UVFw II | TGGTCAAGACCCCCATCTTYAYGYAYAA | 28 |
| UVRv II | CAGATGGTGATGGCGGCYTTNGCDATNC | 28 |
| 3′ RACE LW | tgatgaccatctcgctgtggttct | 24 |
| 5′ RACE LW | aggtctcgtagtagcagttgacg | 23 |
| 3′ RACE UV I | cgaagcaaccagaatcaacagacc | 24 |
| 5′ RACE UV I | tagctccgatacctgataaagagc | 24 |
| 3′ RACE UV II | tattctgcctgctatggaagtct | 23 |
| 5′ RACE UV II | acctcggttgaatgagttgtaga | 23 |
Degenerate primers were named according to the spectral class and their orientation, i.e. LWFw - long wavelength, forward; LWRv - long wavelength, reverse (see Fig. 2). The 3′ RACE primers shown are all forward and the 5′ RACE are all reverse.