Table 1.

Pre-exposure conditions for DSI in Series 2

ConditionsApical solutionsBasolateral solutions
Asymmetrical DSI   
   Control FW CM 
   Amm FW, Amm CM, Amm 
   Cort FW CM, Cort 
   Amm+Cort FW, Amm CM, Amm+Cort 
Symmetrical DSI   
   Control CM CM 
   Amm CM, Amm CM, Amm 
   Cort CM CM, Cort 
   Amm+Cort CM, Amm CM, Amm+Cort 
ConditionsApical solutionsBasolateral solutions
Asymmetrical DSI   
   Control FW CM 
   Amm FW, Amm CM, Amm 
   Cort FW CM, Cort 
   Amm+Cort FW, Amm CM, Amm+Cort 
Symmetrical DSI   
   Control CM CM 
   Amm CM, Amm CM, Amm 
   Cort CM CM, Cort 
   Amm+Cort CM, Amm CM, Amm+Cort 

DSI, double seeded insert; FW, freshwater; CM, culture medium; Amm, 2000μmol l–1 NH4Cl; Cort, 1000 ng ml–1 cortisol

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