The effects of increased acclimation temperature on the numbers and two-dimensional surface areas of individual ionocytes as identified either using osmium-zinc iodide staining or Na+/K+-ATPase immunofluorescence in goldfish (Carassius auratus)
| . | . | 7°C . | 25°C . |
|---|---|---|---|
| Osmium-zinc iodide technique | Average ionocyte surface area (μm2) | 53.2±4.7 | 37.0±2.8* |
| Average number of ionocytes per mm of filament | 101.1±3.2 | 58.0±5.4* | |
| NKA immunofluorescence technique | Average ionocyte surface area (μm2) | 42.1±4.9 | 25.1±3.3* |
| Average number of ionocytes per mm of filament | 96.6±5.2 | 67.1±10.6* |
| . | . | 7°C . | 25°C . |
|---|---|---|---|
| Osmium-zinc iodide technique | Average ionocyte surface area (μm2) | 53.2±4.7 | 37.0±2.8* |
| Average number of ionocytes per mm of filament | 101.1±3.2 | 58.0±5.4* | |
| NKA immunofluorescence technique | Average ionocyte surface area (μm2) | 42.1±4.9 | 25.1±3.3* |
| Average number of ionocytes per mm of filament | 96.6±5.2 | 67.1±10.6* |
NKA, Na+/K+-ATPase
Data are presented as means ± s.e.m. Significant differences from values at 7°C are indicated by asterisks (P<0.05; N=6 for both groups)